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Anchorage-dependent multicellular aggregate formation induces CD44 high cancer stem cell-like ATL cells in an NF-kappa B- and vimentin-dependent manner

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タイトル: Anchorage-dependent multicellular aggregate formation induces CD44 high cancer stem cell-like ATL cells in an NF-kappa B- and vimentin-dependent manner
著者: Miyatake, Yukiko 著作を一覧する
Sheehy, Noreen 著作を一覧する
Ikeshita, Shunji 著作を一覧する
Hall, William W. 著作を一覧する
Kasahara, Masanori 著作を一覧する
キーワード: Cancer stem cells
Coculture
CD44
Vimentin, ATL
発行日: 2015年 2月 1日
出版者: Elsevier Ireland
誌名: Cancer letters
巻: 357
号: 1
開始ページ: 355
終了ページ: 363
出版社 DOI: 10.1016/j.canlet.2014.11.055
抄録: Adult T-cell leukemia/lymphoma (ATL) is an intractable T-cell malignancy accompanied by massive invasion of lymphoma cells into various tissues. We demonstrate here that ATL cells cultured on a layer of epithelial-like feeder cells form anchorage-dependent multicellular aggregates (Ad-MCAs) and that a fraction of MCA-forming ATL cells acquire CD44 high cancer stem cell-like phenotypes. ATL cells forming Ad-MCAs displayed extracellular microvesicles with enhanced expression of CD44v9 at cell synapses, augmented expression of multidrug resistance protein 1, and increased NF-kappa B activity. Blockade of the NF kappa B pathway dramatically reduced Ad-MCA formation by ATL cells and the emergence of CD44 high ATL cells, but left a considerable number of ATL cells adhering to the feeder layer. Disruption of vimentin cytoskeleton by treatment with withaferin A, a natural steroidal lactone, suppressed not only the adhesion of ATL cells to the feeder layer but also subsequent Ad-MCA formation by ATL cells, suggesting the involvement of vimentin in anchoring ATL cells to the feeder layer. Ad-MCA formation by ATL cells on a layer of epithelial-like feeder cells may mimic critical events that occur in metastatic colonization. (c) 2014 Elsevier Ireland Ltd. All rights reserved.
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/58276
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 宮武 由甲子

 

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