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Hokkaido University Collection of Scholarly and Academic Papers >
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Anchorage-dependent multicellular aggregate formation induces CD44 high cancer stem cell-like ATL cells in an NF-kappa B- and vimentin-dependent manner
| Title: | Anchorage-dependent multicellular aggregate formation induces CD44 high cancer stem cell-like ATL cells in an NF-kappa B- and vimentin-dependent manner |
| Authors: | Miyatake, Yukiko Browse this author →KAKEN DB | | Sheehy, Noreen Browse this author | | Ikeshita, Shunji Browse this author | | Hall, William W. Browse this author | | Kasahara, Masanori Browse this author →KAKEN DB |
| Keywords: | Cancer stem cells | | Coculture | | CD44 | | Vimentin, ATL |
| Issue Date: | 1-Feb-2015 |
| Publisher: | Elsevier Ireland |
| Journal Title: | Cancer letters |
| Volume: | 357 |
| Issue: | 1 |
| Start Page: | 355 |
| End Page: | 363 |
| Publisher DOI: | 10.1016/j.canlet.2014.11.055 |
| PMID: | 25448402 |
| Abstract: | Adult T-cell leukemia/lymphoma (ATL) is an intractable T-cell malignancy accompanied by massive invasion of lymphoma cells into various tissues. We demonstrate here that ATL cells cultured on a layer of epithelial-like feeder cells form anchorage-dependent multicellular aggregates (Ad-MCAs) and that a fraction of MCA-forming ATL cells acquire CD44 high cancer stem cell-like phenotypes. ATL cells forming Ad-MCAs displayed extracellular microvesicles with enhanced expression of CD44v9 at cell synapses, augmented expression of multidrug resistance protein 1, and increased NF-kappa B activity. Blockade of the NF kappa B pathway dramatically reduced Ad-MCA formation by ATL cells and the emergence of CD44 high ATL cells, but left a considerable number of ATL cells adhering to the feeder layer. Disruption of vimentin cytoskeleton by treatment with withaferin A, a natural steroidal lactone, suppressed not only the adhesion of ATL cells to the feeder layer but also subsequent Ad-MCA formation by ATL cells, suggesting the involvement of vimentin in anchoring ATL cells to the feeder layer. Ad-MCA formation by ATL cells on a layer of epithelial-like feeder cells may mimic critical events that occur in metastatic colonization. (c) 2014 Elsevier Ireland Ltd. All rights reserved. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/58276 |
| Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 宮武 由甲子
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