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Influence of the pattern size of micropatterned scaffolds on cell morphology, proliferation, migration and F-actin expression

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/58466

Title: Influence of the pattern size of micropatterned scaffolds on cell morphology, proliferation, migration and F-actin expression
Authors: Sunami, Hiroshi Browse this author →KAKEN DB
Yokota, Ikuko Browse this author
Igarashi, Yasuyuki Browse this author →KAKEN DB
Issue Date: 1-Mar-2014
Publisher: Royal Society of Chemistry
Journal Title: Biomaterials science
Volume: 2
Issue: 3
Start Page: 399
End Page: 409
Publisher DOI: 10.1039/c3bm60237k
Abstract: To determine how the three-dimensional (3D) shape of scaffolds influences cell functions, 3D micropatterned scaffolds of various sizes were fabricated on a silicon substrate. The micropatterns were equilateral triangular pores with 3-20 mu m long sides, and all had the same pore ratio (total pore area per unit area) and depth. The patterns only differed in terms of their 2D size. Such scaffolds have not been previously generated, and thus the effects of pattern size on cell functions have not been addressed. NIH-3T3 cells were cultured on these micropatterned scaffolds, and their morphology, proliferation rate, migration rate, and level of F-actin expression were assessed. Cells became more rounded and F-actin expression decreased as the pattern size of the scaffold decreased. Relationships were also demonstrated between pattern size and cell proliferation and migration. These results suggest that the pattern size of 3D micropatterned scaffolds affects the level of mechanical stress that cells experience, and thereby influences F-actin expression, cell morphology, cell proliferation and cell migration.
Type: article (author version)
URI: http://hdl.handle.net/2115/58466
Appears in Collections:生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 五十嵐 靖之

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