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Population genetics of Leishmania (Leishmania) major DNA isolated from cutaneous leishmaniasis patients in Pakistan based on multilocus microsatellite typing

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Title: Population genetics of Leishmania (Leishmania) major DNA isolated from cutaneous leishmaniasis patients in Pakistan based on multilocus microsatellite typing
Authors: Alam, Mohammad Zahangir Browse this author
Bhutto, Abdul Manan Browse this author
Soomro, Farooq Rahman Browse this author
Baloch, Javed Hussain Browse this author
Nakao, Ryo Browse this author →KAKEN DB
Kato, Hirotomo Browse this author →KAKEN DB
Schoenian, Gabriele Browse this author
Uezato, Hiroshi Browse this author →KAKEN DB
Hashiguchi, Yoshihisa Browse this author →KAKEN DB
Katakura, Ken Browse this author →KAKEN DB
Keywords: Leishmania (Leishmania) major
Microsatellite typing
Population genetics
Issue Date: 16-Jul-2014
Publisher: Biomed Central
Journal Title: Parasites & Vectors
Volume: 7
Start Page: 332
Publisher DOI: 10.1186/1756-3305-7-332
PMID: 25030377
Abstract: Background: Cutaneous leishmaniasis (CL) is a major and fast increasing public health problem, both among the local Pakistani populations and the Afghan refugees in camps. Leishmania (Leishmania) major is one of the etiological agents responsible for CL in Pakistan. Genetic variability and population structure have been investigated for 66 DNA samples of L. (L.) major isolated from skin biopsy of CL patients. Methods: Multilocus microsatellite typing (MLMT), employing 10 independent genetic markers specific to L. (L.) major, was used to investigate the genetic polymorphisms and population structures of Pakistani L. (L.) major DNA isolated from CL human cases. Their microsatellite profiles were compared to those of 130 previously typed strains of L. (L.) major from various geographical localities. Results: All the markers were polymorphic and fifty-one MLMT profiles were recognized among the 66 L. (L.) major DNA samples. The data displayed significant microsatellite polymorphisms with rare allelic heterozygosities. A Bayesian model-based approach and phylogenetic analysis inferred two L. (L.) major populations in Pakistan. Thirty-four samples belonged to one population and the remaining 32 L. (L.) major samples grouped together into another population. The two Pakistani L. (L.) major populations formed separate clusters, which differ genetically from the populations of L. (L.) major from Central Asia, Iran, Middle East and Africa. Conclusions: The considerable genetic variability of L. (L.) major might be related to the existence of different species of sand fly and/or rodent reservoir host in Sindh province, Pakistan. A comprehensive study of the epidemiology of CL including the situation or spreading of reservoirs and sand fly vectors in these foci is, therefore, warranted.
Type: article
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 片倉 賢

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