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Gene expression profile of bovine bone marrow mesenchymal stem cell during spontaneous chondrogenic differentiation in pellet culture system

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Title: Gene expression profile of bovine bone marrow mesenchymal stem cell during spontaneous chondrogenic differentiation in pellet culture system
Authors: Bosnakovski, Darko Browse this author
Mizuno, Morimichi Browse this author
Kim, Gonhyung Browse this author
Takagi, Satoshi Browse this author
Okumura, Masahiro Browse this author
Fujinaga, Toru Browse this author
Keywords: bovine
mesenchymal stem cell
pellet culture
Issue Date: 28-Feb-2006
Publisher: The Graduate School of Veterinary Medicine, Hokkaido University
Journal Title: Japanese Journal of Veterinary Research
Volume: 53
Issue: 3-4
Start Page: 127
End Page: 139
Abstract: Bovine bone marrow mesenchymal stem cells(MSCs)cultured in condensate culture, spontaneous and independent for any external biostimulants, undergo chondrogenic differentiation. In the present study, the bovine MSC chondrogenesis pathway was studied by analyzing stage-specific gene expression using quantitative“Real Time”reverse transcriptase polymerase chain reaction(qRT-PCR).Results showed that bovine MSCs underwent complete chondrogenesis ; the initial stage was characterized by expression of sox9 messenger ribonucleic acid(mRNA),followed by high transcription of chondrocyte specific genes, collagen type II and IX, biglycan and cartilage oligomeric matrix protein, and the final prehypertrophic and/or hypertrophic stage was distinguished by increased expression of collagen type X. From day 7to day14of differentiation increased mRNA expression of the transforming growth factors β1and β2,basic fibroblast growth factor(FGF 2),bone morphogenic protein6(BMP 6),insulin-like growth factors1,parathyroid hormone related peptide and indian hedgehog(Ihh)were detected. These results suggest that these well know chondrogenic growth factors may play a role in bovine chondrogenesis in autocrine and/or paracrine manner. On day21of the culture, FGF 2,BMP 6 and Ihh were highly expressed, compared to cells culextertured in monolayer manner, which suggests a possible function in maintaining the terminal stage of differentiation. This data extends our knowledge about the unusual species-specific bovine MSC chondrogenesis, allowing us to define the phenotype of the differentiated cells. Furthermore, this study contributes to our in understanding of known chondrogenic-growth factors in autocrine and/or paracrine manner playing a role in the spontaneous differentiation.
Type: bulletin (article)
Appears in Collections:Japanese Journal of Veterinary Research > Volume 53, Number 3-4

Submitter: 獣医学部図書室

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