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Cloning and expression of three ladA-type alkane monooxygenase genes from an extremely thermophilic alkane-degrading bacterium Geobacillus thermoleovorans B23

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Title: Cloning and expression of three ladA-type alkane monooxygenase genes from an extremely thermophilic alkane-degrading bacterium Geobacillus thermoleovorans B23
Authors: Boonmak, Chanita Browse this author
Takahashi, Yasunori Browse this author
Morikawa, Masaaki Browse this author →KAKEN DB
Keywords: LadA alkane monooxygenase
Geobacillus thermoleovorans B23
Alkane degradation
Genome
Issue Date: May-2014
Publisher: Springer Japan
Journal Title: Extremophiles
Volume: 18
Issue: 3
Start Page: 515
End Page: 523
Publisher DOI: 10.1007/s00792-014-0636-y
PMID: 24682607
Abstract: An extremely thermophilic bacterium, Geobacillus thermoleovorans B23, is capable of degrading a broad range of alkanes (with carbon chain lengths ranging between C11 and C32) at 70 A degrees C. Whole-genome sequence analysis revealed that unlike most alkane-degrading bacteria, strain B23 does not possess an alkB-type alkane monooxygenase gene. Instead, it possesses a cluster of three ladA-type genes, ladA alpha(B23), ladA beta(B23), and ladB (B23), on its chromosome, whose protein products share significant amino acid sequence identities, 49.8, 34.4, and 22.7 %, respectively, with that of ladA alkane monooxygenase gene found on a plasmid of Geobacillus thermodetrificans NG 80-2. Each of the three genes, ladA alpha(B23), ladA beta(B23), and ladB (B23), was heterologously expressed individually in an alkB1 deletion mutant strain, Pseudomonas fluorescens KOB2 Delta 1. It was found that all three genes were functional in P. fluorescens KOB2 Delta 1, and partially restored alkane degradation activity. In this study, we suggest that G. thermoleovorans B23 utilizes multiple LadA-type alkane monooxygenases for the degradation of a broad range of alkanes.
Rights: The final publication is available at Springer via http://dx.doi.org/10.1007/s00792-014-0636-y
Type: article (author version)
URI: http://hdl.handle.net/2115/59072
Appears in Collections:環境科学院・地球環境科学研究院 (Graduate School of Environmental Science / Faculty of Environmental Earth Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 森川 正章

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