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1壁性骨欠損における歯根象牙質表面へのBMP-2塗布による歯周組織再生効果

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Title: 1壁性骨欠損における歯根象牙質表面へのBMP-2塗布による歯周組織再生効果
Other Titles: Effect of BMP-2 application to root dentin surface on periodontal regeneration in 1-wall infrabony defects
Authors: 加藤, 昭人1 Browse this author →KAKEN DB
宮治, 裕史2 Browse this author →KAKEN DB
菅谷, 勉3 Browse this author →KAKEN DB
川浪, 雅光4 Browse this author →KAKEN DB
Authors(alt): Kato, Akihito1
Miyaji, Hirofumi2
Sugaya, Tsutomu3
Kawanami, Masamitsu4
Keywords: BMP-2
歯周組織再生
1壁性骨欠損
periodontal regeneration
1-wall infrabony defects
Issue Date: 28-Dec-2007
Publisher: 日本歯周病学会
Journal Title: Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology)
Volume: 49
Issue: 4
Start Page: 296
End Page: 304
Publisher DOI: 10.2329/perio.49.296
Abstract:  本研究の目的は, 1壁性骨欠損における歯根象牙質表面へのBMP-2の塗布が, 歯周組織再生に及ぼす効果を評価することである。  実験動物にはビーグル犬7頭を使用した。先に下顎第一前臼歯と第三前臼歯の抜歯を行い, 8週後に下顎第二前臼歯近心根と遠心根, および第四前臼歯近心根の歯肉歯槽粘膜を部分層弁剥離して骨膜を除去し, 高さ5mm, 近遠心幅3mmの1壁性骨欠損を作製した。露出した根面の歯根膜とセメント質を除去して, 根面を24%EDTAで3分間脱灰後, BMP群ではBMP-2(1000μg/m)を根面に塗布し, 未塗布群ではBMP-2を塗布せずに歯肉歯槽粘膜弁を復位縫合した。観察期間は4週および8週とし, 病理組織学的観察と組織学的計測を行った。  その結果, 8週において歯槽骨新生率は, 未塗布群32.2±15.1%, BMP群70.0±12.9%, セメント質新生率は, 未塗布群13.4±10.6%, BMP群45.4±10.0%, 骨性癒着率は, 未塗布群0%, BMP群13.1±10.0%で, いずれもBMP群は有意に大きかった(p<0.01)。接合上皮深部増殖率は, 未塗布群8.4±10.0%, BMP群1.3±3.1%で, BMP群は有意に小さかった(p<0.05)。 以上の結果から, 1壁性骨欠損においてBMP-2を根面に塗布することは, 歯周組織再生に有効であることが示唆された。
This study evaluated periodontal regeneration after the application of BMP-2 to root dentin surface in 1-wall infrabony defects. The mandibular first and third premolars in seven beagle dogs were extracted prior to experimental surgery. Eight weeks after the extraction, the periosteum was removed from the alveolar bone following the retraction of a partial thickness of the muco-gingival flap. Subsequently, 1-wall infrabony defects (5 mm in height, 3 mm in width) were surgically created in the mesial and distal sides of the mandibular second premolars, and in the mesial sides of the mandibular fourth premolars. Then, the periodontal ligament and the cementum on the exposed root surface were thoroughly removed. The denuded root surface was demineralized with 24% EDTA for 3 minutes. In the BMP group, BMP-2 solution (1000 μg/ml) was applied to the root surface, and the flap was repositioned. BMP-2 was not applied in the control group. Histological and histometrical analyses were performed at four and eight weeks after surgery. At eight weeks,new bone formation, new cementum formation and ankylosis were significantly greater in the BMP group than in the control group (p < 0.01). The down-growth of the junctional epithelium in the BMP group was significantly smaller than that in the control group (p < 0.05). These findings suggest that periodontal regeneration was enhanced by the application of BMP-2 to the root dentin surface in 1-wall infrabony defects.
Type: article
URI: http://hdl.handle.net/2115/59126
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 加藤 昭人

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