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Effect of Tinospora cordifolia on the reduction of ultraviolet radiation-induced cytotoxicity and DNA damage in PC12 cells

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Title: Effect of Tinospora cordifolia on the reduction of ultraviolet radiation-induced cytotoxicity and DNA damage in PC12 cells
Authors: Masuma, Runa Browse this author
Okuno, Tsutomu Browse this author
Choudhuri, Mohammad Shahabuddin Kabir Browse this author
Saito, Takeshi Browse this author →KAKEN DB
Kurasaki, Masaaki Browse this author →KAKEN DB
Keywords: Cell viability
cyclobutane pyrimidine dimer
DNA damage
UV radiation
Issue Date: 3-Jun-2014
Publisher: Taylor & Francis
Journal Title: Journal of Environmental Science and Health, Part B : Pesticides Food Contaminants and Agricultural Wastes
Volume: 49
Issue: 6
Start Page: 416
End Page: 421
Publisher DOI: 10.1080/03601234.2014.894777
PMID: 24762179
Abstract: The safety of Tinospora cordifolia and its potential to protect against ultraviolet radiation-induced cytotoxicity and DNA damage in PC12 cells were investigated. To evaluate the safety of T. cordifolia, cell viability and agarose gel electrophoresis were carried out using PC12 cells treated with 0 to 100g mL(-1) of methanol extract of T. cordifolia. T. cordifolia extracts did not show cytotoxicity ranging 0 to 100g mL(-1). In addition, T. cordifolia extracts significantly increased cell viability at 1ng, 10ng and 1g mL(-1) concentrations in serum-deprived medium compared to control. To confirm the protective role against UV-induced damage, PC12 cells alone or in the presence of 10ng, 100ng, or 1g mL(-1) of T. cordifolia extract were exposed to 250, 270 and 290nm of UV radiation, which corresponded to doses of 120, 150 and 300 mJ cm(-2), respectively. Treatment with T. cordifolia extracts significantly increased the cell survival rate irradiated at 290nm. In addition, T. cordifolia extracts significantly reduced cyclobutane pyrimidine dimer formation induced by UV irradiation at all wavelengths. In conclusion, T. cordifolia is not toxic and safe for cells. Our findings can support its application as phototherapy in the medical sector.
Rights: This is an Author's Accepted Manuscript of an article published in Journal of Environmental Science and Health, Part B Pesticides, Food Contaminants, and Agricultural Wastes, Vol. 49, Iss. 6, 2014, copyright Taylor & Francis, available online at: http://www.tandfonline.com/10.1080/03601234.2014.894777
Type: article (author version)
URI: http://hdl.handle.net/2115/59169
Appears in Collections:環境科学院・地球環境科学研究院 (Graduate School of Environmental Science / Faculty of Environmental Earth Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 藏崎 正明

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