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Hokkaido University Collection of Scholarly and Academic Papers >
獣医学研究科 >
Japanese Journal of Veterinary Research >
Volume 53, Number 3-4 >
Alymphoplasia mice are resistant to prion infection via oral route
| タイトル: | Alymphoplasia mice are resistant to prion infection via oral route |
| 著者: | Horiuchi, Motohiro 著作を一覧する
Furuoka, Hidefumi 著作を一覧する
Kitamura, Nobuo 著作を一覧する
Shinagawa, Morikazu 著作を一覧する |
| キーワード: | prion
scrapie
alymphoplasia
GALT |
| 発行日: | 2006年 2月28日 |
| 出版者: | The Graduate School of Veterinary Medicine, Hokkaido University |
| 誌名: | Japanese Journal of Veterinary Research |
| 巻: | 53 |
| 号: | 3-4 |
| 開始ページ: | 149 |
| 終了ページ: | 157 |
| 抄録: | The major cause of infection in animal prion diseases is thought to be consumption
of prion-contaminated stuff. There is evidence that the enteric
nerve system (ENS) and gut-associated lymphoid tissues (GATL) are involved
in the establishment of prion infection through alimentary tract. To elucidate
the initial entry port for prion, we inoculated prion to alymphoplasia (aly )
mice showing a deficiency in systemic lymph nodes and Peyer’s patches. The
aly ／aly mice were susceptible to prion infection by intra-cranial inoculation
and there were no differences in incubation periods between aly／aly mice and
wild-type C５７BL／６J mice. Incubation periods in aly／aly mice were about２０
days longer than those in C５７BL／６J mice with the intra-peritoneal inoculation.
The aly ／aly mice were completely resistant to prion infection by per os
administration, while C５７BL／６J mice were sensitive as they entered the terminal
stage of disease around３００days post inoculation. PrPSc were detected in
the intestine and spleen of C５７BL／６J mice inoculated with prion intraperitoneally
or orally ; however PrPSc was not detected in the spleen and intestine
of aly ／aly mice. Prion infectivity was detected in the intestines and
spleens of prion-inoculated C５７BL／６J mice, even after the early stages of exposure,
while no infectivity was detected in these tissues of prion-inoculated
aly ／aly mice. No apparent differences were observed in the organization of
the enteric nerve system between wild-type and aly ／aly mice. These results
indicate that GALT rather than ENS acts as the primary entry port for prion
after oral exposure.
１）Laboratory of Prion Diseases, Graduate |
| 資料タイプ: | bulletin |
| URI: | http://hdl.handle.net/2115/5935 |
| 出現コレクション: | Volume 53, Number 3-4
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提供者: 堀内 基広
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