HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Japanese Journal of Veterinary Research >
Volume 53, Number 3-4 >

Alymphoplasia mice are resistant to prion infection via oral route

Files in This Item:
53(3-4)-3.pdf345.02 kBPDFView/Open
Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.53.3-4.149

Title: Alymphoplasia mice are resistant to prion infection via oral route
Authors: Horiuchi, Motohiro Browse this author
Furuoka, Hidefumi Browse this author
Kitamura, Nobuo Browse this author
Shinagawa, Morikazu Browse this author
Keywords: prion
scrapie
alymphoplasia
GALT
Issue Date: 28-Feb-2006
Publisher: The Graduate School of Veterinary Medicine, Hokkaido University
Journal Title: Japanese Journal of Veterinary Research
Volume: 53
Issue: 3-4
Start Page: 149
End Page: 157
Abstract: The major cause of infection in animal prion diseases is thought to be consumption of prion-contaminated stuff. There is evidence that the enteric nerve system (ENS) and gut-associated lymphoid tissues (GATL) are involved in the establishment of prion infection through alimentary tract. To elucidate the initial entry port for prion, we inoculated prion to alymphoplasia (aly ) mice showing a deficiency in systemic lymph nodes and Peyer’s patches. The aly /aly mice were susceptible to prion infection by intra-cranial inoculation and there were no differences in incubation periods between aly/aly mice and wild-type C57BL/6J mice. Incubation periods in aly/aly mice were about20 days longer than those in C57BL/6J mice with the intra-peritoneal inoculation. The aly /aly mice were completely resistant to prion infection by per os administration, while C57BL/6J mice were sensitive as they entered the terminal stage of disease around300days post inoculation. PrPSc were detected in the intestine and spleen of C57BL/6J mice inoculated with prion intraperitoneally or orally ; however PrPSc was not detected in the spleen and intestine of aly /aly mice. Prion infectivity was detected in the intestines and spleens of prion-inoculated C57BL/6J mice, even after the early stages of exposure, while no infectivity was detected in these tissues of prion-inoculated aly /aly mice. No apparent differences were observed in the organization of the enteric nerve system between wild-type and aly /aly mice. These results indicate that GALT rather than ENS acts as the primary entry port for prion after oral exposure. 1)Laboratory of Prion Diseases, Graduate
Type: bulletin (article)
URI: http://hdl.handle.net/2115/5935
Appears in Collections:Japanese Journal of Veterinary Research > Volume 53, Number 3-4

Submitter: 堀内 基広

Export metadata:

OAI-PMH ( junii2 , jpcoar )

MathJax is now OFF:


 

 - Hokkaido University