Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Japanese Journal of Veterinary Research >
Volume 53, Number 3-4 >
Alymphoplasia mice are resistant to prion infection via oral route
Title: | Alymphoplasia mice are resistant to prion infection via oral route |
Authors: | Horiuchi, Motohiro Browse this author | Furuoka, Hidefumi Browse this author | Kitamura, Nobuo Browse this author | Shinagawa, Morikazu Browse this author |
Keywords: | prion | scrapie | alymphoplasia | GALT |
Issue Date: | 28-Feb-2006 |
Publisher: | The Graduate School of Veterinary Medicine, Hokkaido University |
Journal Title: | Japanese Journal of Veterinary Research |
Volume: | 53 |
Issue: | 3-4 |
Start Page: | 149 |
End Page: | 157 |
Abstract: | The major cause of infection in animal prion diseases is thought to be consumption of prion-contaminated stuff. There is evidence that the enteric nerve system (ENS) and gut-associated lymphoid tissues (GATL) are involved in the establishment of prion infection through alimentary tract. To elucidate the initial entry port for prion, we inoculated prion to alymphoplasia (aly ) mice showing a deficiency in systemic lymph nodes and Peyer’s patches. The aly /aly mice were susceptible to prion infection by intra-cranial inoculation and there were no differences in incubation periods between aly/aly mice and wild-type C57BL/6J mice. Incubation periods in aly/aly mice were about20 days longer than those in C57BL/6J mice with the intra-peritoneal inoculation. The aly /aly mice were completely resistant to prion infection by per os administration, while C57BL/6J mice were sensitive as they entered the terminal stage of disease around300days post inoculation. PrPSc were detected in the intestine and spleen of C57BL/6J mice inoculated with prion intraperitoneally or orally ; however PrPSc was not detected in the spleen and intestine of aly /aly mice. Prion infectivity was detected in the intestines and spleens of prion-inoculated C57BL/6J mice, even after the early stages of exposure, while no infectivity was detected in these tissues of prion-inoculated aly /aly mice. No apparent differences were observed in the organization of the enteric nerve system between wild-type and aly /aly mice. These results indicate that GALT rather than ENS acts as the primary entry port for prion after oral exposure. 1)Laboratory of Prion Diseases, Graduate |
Type: | bulletin (article) |
URI: | http://hdl.handle.net/2115/5935 |
Appears in Collections: | Japanese Journal of Veterinary Research > Volume 53, Number 3-4
|
Submitter: 堀内 基広
|