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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Japanese Journal of Veterinary Research >
Volume 53, Number 3-4 >
Alymphoplasia mice are resistant to prion infection via oral route
| Title: | Alymphoplasia mice are resistant to prion infection via oral route |
| Authors: | Horiuchi, Motohiro Browse this author | | Furuoka, Hidefumi Browse this author | | Kitamura, Nobuo Browse this author | | Shinagawa, Morikazu Browse this author |
| Keywords: | prion | | scrapie | | alymphoplasia | | GALT |
| Issue Date: | 28-Feb-2006 |
| Publisher: | The Graduate School of Veterinary Medicine, Hokkaido University |
| Journal Title: | Japanese Journal of Veterinary Research |
| Volume: | 53 |
| Issue: | 3-4 |
| Start Page: | 149 |
| End Page: | 157 |
| Abstract: | The major cause of infection in animal prion diseases is thought to be consumption of prion-contaminated stuff. There is evidence that the enteric nerve system (ENS) and gut-associated lymphoid tissues (GATL) are involved in the establishment of prion infection through alimentary tract. To elucidate the initial entry port for prion, we inoculated prion to alymphoplasia (aly ) mice showing a deficiency in systemic lymph nodes and Peyer’s patches. The aly ／aly mice were susceptible to prion infection by intra-cranial inoculation and there were no differences in incubation periods between aly／aly mice and wild-type C５７BL／６J mice. Incubation periods in aly／aly mice were about２０ days longer than those in C５７BL／６J mice with the intra-peritoneal inoculation. The aly ／aly mice were completely resistant to prion infection by per os administration, while C５７BL／６J mice were sensitive as they entered the terminal stage of disease around３００days post inoculation. PrPSc were detected in the intestine and spleen of C５７BL／６J mice inoculated with prion intraperitoneally or orally ; however PrPSc was not detected in the spleen and intestine of aly ／aly mice. Prion infectivity was detected in the intestines and spleens of prion-inoculated C５７BL／６J mice, even after the early stages of exposure, while no infectivity was detected in these tissues of prion-inoculated aly ／aly mice. No apparent differences were observed in the organization of the enteric nerve system between wild-type and aly ／aly mice. These results indicate that GALT rather than ENS acts as the primary entry port for prion after oral exposure. １）Laboratory of Prion Diseases, Graduate |
| Type: | bulletin (article) |
| URI: | http://hdl.handle.net/2115/5935 |
| Appears in Collections: | Japanese Journal of Veterinary Research > Volume 53, Number 3-4
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Submitter: 堀内 基広
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