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The E3 ubiquitin ligase TRIM23 regulates adipocyte differentiation via stabilization of the adipogenic activator PPARγ

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Title: The E3 ubiquitin ligase TRIM23 regulates adipocyte differentiation via stabilization of the adipogenic activator PPARγ
Authors: Watanabe, Masashi Browse this author
Takahashi, Hidehisa Browse this author →KAKEN DB
Saeki, Yasushi Browse this author →KAKEN DB
Ozaki, Takashi Browse this author
Itoh, Shihori Browse this author
Suzuki, Masanobu Browse this author
Mizushima, Wataru Browse this author
Tanaka, Keiji Browse this author →KAKEN DB
Hatakeyama, Shigetsugu Browse this author →KAKEN DB
Issue Date: 23-Apr-2015
Publisher: eLife Sciences Publications
Journal Title: Elife
Volume: 4
Start Page: e05615
Publisher DOI: 10.7554/eLife.05615
Abstract: Adipocyte differentiation is a strictly controlled process regulated by a series of transcriptional activators. Adipogenic signals activate early adipogenic activators and facilitate the transient formation of early enhanceosomes at target genes. These enhancer regions are subsequently inherited by late enhanceosomes. PPAR gamma is one of the late adipogenic activators and is known as a master regulator of adipogenesis. However, the factors that regulate PPAR gamma expression remain to be elucidated. Here, we show that a novel ubiquitin E3 ligase, tripartite motif protein 23 (TRIM23), stabilizes PPAR gamma protein and mediates atypical polyubiquitin conjugation. TRIM23 knockdown caused a marked decrease in PPAR gamma protein abundance during preadipocyte differentiation, resulting in a severe defect in late adipogenic differentiation, whereas it did not affect the formation of early enhanceosomes. Our results suggest that TRIM23 plays a critical role in the switching from early to late adipogenic enhanceosomes by stabilizing PPAR gamma protein possibly via atypical polyubiquitin conjugation.
Type: article
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 畠山 鎮次

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