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The E3 ubiquitin ligase TRIM23 regulates adipocyte differentiation via stabilization of the adipogenic activator PPARγ
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Title: | The E3 ubiquitin ligase TRIM23 regulates adipocyte differentiation via stabilization of the adipogenic activator PPARγ |
Authors: | Watanabe, Masashi Browse this author | Takahashi, Hidehisa Browse this author →KAKEN DB | Saeki, Yasushi Browse this author →KAKEN DB | Ozaki, Takashi Browse this author | Itoh, Shihori Browse this author | Suzuki, Masanobu Browse this author | Mizushima, Wataru Browse this author | Tanaka, Keiji Browse this author →KAKEN DB | Hatakeyama, Shigetsugu Browse this author →KAKEN DB |
Issue Date: | 23-Apr-2015 |
Publisher: | eLife Sciences Publications |
Journal Title: | Elife |
Volume: | 4 |
Start Page: | e05615 |
Publisher DOI: | 10.7554/eLife.05615 |
Abstract: | Adipocyte differentiation is a strictly controlled process regulated by a series of transcriptional activators. Adipogenic signals activate early adipogenic activators and facilitate the transient formation of early enhanceosomes at target genes. These enhancer regions are subsequently inherited by late enhanceosomes. PPAR gamma is one of the late adipogenic activators and is known as a master regulator of adipogenesis. However, the factors that regulate PPAR gamma expression remain to be elucidated. Here, we show that a novel ubiquitin E3 ligase, tripartite motif protein 23 (TRIM23), stabilizes PPAR gamma protein and mediates atypical polyubiquitin conjugation. TRIM23 knockdown caused a marked decrease in PPAR gamma protein abundance during preadipocyte differentiation, resulting in a severe defect in late adipogenic differentiation, whereas it did not affect the formation of early enhanceosomes. Our results suggest that TRIM23 plays a critical role in the switching from early to late adipogenic enhanceosomes by stabilizing PPAR gamma protein possibly via atypical polyubiquitin conjugation. |
Rights: | http://creativecommons.org/licenses/by/4.0/ |
Type: | article |
URI: | http://hdl.handle.net/2115/59433 |
Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 畠山 鎮次
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