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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Peer-reviewed Journal Articles, etc >
Addition of D-penicillamine, hypotaurine, and epinephrine (PHE) mixture to IVF medium maintains motility and longevity of bovine sperm and enhances stable production of blastocysts in vitro
| Title: | Addition of D-penicillamine, hypotaurine, and epinephrine (PHE) mixture to IVF medium maintains motility and longevity of bovine sperm and enhances stable production of blastocysts in vitro |
| Authors: | KANG, Sung-Sik Browse this author | | KOYAMA, Keisuke Browse this author | | HUANG, Weiping Browse this author | | YANG, Yinghua Browse this author | | YANAGAWA, Yojiro Browse this author | | TAKAHASHI, Yoshiyuki Browse this author →KAKEN DB | | NAGANO, Masashi Browse this author →KAKEN DB |
| Keywords: | computer-assisted sperm analysis (CASA) | | In vitro fertilization (IVF) | | Oocyte | | PHE | | Theophylline |
| Issue Date: | Apr-2015 |
| Publisher: | 日本繁殖生物学会 |
| Journal Title: | Journal of Reproduction and Development |
| Volume: | 61 |
| Issue: | 2 |
| Start Page: | 99 |
| End Page: | 105 |
| Publisher DOI: | 10.1262/jrd.2014-112 |
| Abstract: | The present study aimed to establish an efficient system for bovine embryo production by in vitro fertilization (IVF) that can achieve stable normal fertilization and blastocyst developmental rates in any bull without optimization of the sperm concentration in IVF medium. We examined the effects of a PHE mixture (20 μM D-penicillamine, 10 μM hypotaurine and 1 μM epinephrine), theophylline (2.5 mM), and sperm concentration (1, 2 or 5 × 106 cells/ml) on fertilization and blastocyst developmental rates. High cleavage rates (78.3 to 92.4%) and blastocyst developmental rates (31.9 to 62.0%) at day 7 were obtained in the presence of PHE and theophylline in IVF medium with a sperm concentration of 2 × 106 cells/ml using sperm from 9 bulls. In addition, the synergistic effect of PHE and theophylline on normal fertilization (2 pronuclei) was clarified at 12 h after IVF with a sperm concentration of 1 × 106 cells/ml. Moreover, high linearity, high flagellar beat cross frequency, and low amplitude of lateral head of motile sperm were found by computer-assisted sperm analysis. In conclusion, the combination of the PHE mixture and theophylline synergistically accelerates sperm motility and sperm penetration of bovine oocytes. Theophylline activates sperm motility with increasing intracellular cAMP. However, PHE prevents an excessive increase of cAMP and maintains sperm motility without hyperactivation. When the combination of PHE and theophylline is added to IVF medium at a sperm concentration of 2 × 106 cells/ml, we can achieve stable normal fertilization and blastocyst development in any bull. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/59452 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 永野 昌志
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