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Interaction between TIM-1 and NPC1 Is Important for Cellular Entry of Ebola Virus

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/59504

Title: Interaction between TIM-1 and NPC1 Is Important for Cellular Entry of Ebola Virus
Authors: Kuroda, Makoto Browse this author
Fujikura, Daisuke Browse this author →KAKEN DB
Nanbo, Asuka Browse this author →KAKEN DB
Marzi, Andrea Browse this author
Noyori, Osamu Browse this author
Kajihara, Masahiro Browse this author
Maruyama, Junki Browse this author
Matsuno, Keita Browse this author
Miyamoto, Hiroko Browse this author
Yoshida, Reiko Browse this author
Feldmann, Heinz Browse this author
Takada, Ayato Browse this author →KAKEN DB
Issue Date: Jun-2015
Publisher: American Society for Microbiology
Journal Title: Journal of virology
Volume: 89
Issue: 12
Start Page: 6481
End Page: 6493
Publisher DOI: 10.1128/JVI.03156-14
Abstract: Multiple host molecules are known to be involved in the cellular entry of filoviruses, including Ebola virus (EBOV); T-cell immunoglobulin and mucin domain 1 (TIM-1) and Niemann-Pick C1 (NPC1) have been identified as attachment and fusion receptors, respectively. However, the molecular mechanisms underlying the entry process have not been fully understood. We found that TIM-1 and NPC1 colocalized and interacted in the intracellular vesicles where EBOV glycoprotein (GP)-mediated membrane fusion occurred. Interestingly, a TIM-1-specific monoclonal antibody (MAb), M224/1, prevented GP-mediated membrane fusion and also interfered with the binding of TIM-1 to NPC1, suggesting that the interaction between TIM-1 and NPC1 is important for filovirus membrane fusion. Moreover, MAb M224/1 efficiently inhibited the cellular entry of viruses from all known filovirus species. These data suggest a novel mechanism underlying filovirus membrane fusion and provide a potential cellular target for antiviral compounds that can be universally used against filovirus infections.
Type: article
URI: http://hdl.handle.net/2115/59504
Appears in Collections:人獣共通感染症リサーチセンター (Research Center for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 高田 礼人

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