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Interaction between TIM-1 and NPC1 Is Important for Cellular Entry of Ebola Virus
| Title: | Interaction between TIM-1 and NPC1 Is Important for Cellular Entry of Ebola Virus |
| Authors: | Kuroda, Makoto Browse this author | | Fujikura, Daisuke Browse this author →KAKEN DB | | Nanbo, Asuka Browse this author →KAKEN DB | | Marzi, Andrea Browse this author | | Noyori, Osamu Browse this author | | Kajihara, Masahiro Browse this author | | Maruyama, Junki Browse this author | | Matsuno, Keita Browse this author | | Miyamoto, Hiroko Browse this author | | Yoshida, Reiko Browse this author | | Feldmann, Heinz Browse this author | | Takada, Ayato Browse this author →KAKEN DB |
| Issue Date: | Jun-2015 |
| Publisher: | American Society for Microbiology |
| Journal Title: | Journal of virology |
| Volume: | 89 |
| Issue: | 12 |
| Start Page: | 6481 |
| End Page: | 6493 |
| Publisher DOI: | 10.1128/JVI.03156-14 |
| Abstract: | Multiple host molecules are known to be involved in the cellular entry of filoviruses, including Ebola virus (EBOV); T-cell immunoglobulin and mucin domain 1 (TIM-1) and Niemann-Pick C1 (NPC1) have been identified as attachment and fusion receptors, respectively. However, the molecular mechanisms underlying the entry process have not been fully understood. We found that TIM-1 and NPC1 colocalized and interacted in the intracellular vesicles where EBOV glycoprotein (GP)-mediated membrane fusion occurred. Interestingly, a TIM-1-specific monoclonal antibody (MAb), M224/1, prevented GP-mediated membrane fusion and also interfered with the binding of TIM-1 to NPC1, suggesting that the interaction between TIM-1 and NPC1 is important for filovirus membrane fusion. Moreover, MAb M224/1 efficiently inhibited the cellular entry of viruses from all known filovirus species. These data suggest a novel mechanism underlying filovirus membrane fusion and provide a potential cellular target for antiviral compounds that can be universally used against filovirus infections. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/59504 |
| Appears in Collections: | 人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 高田 礼人
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