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MED26 regulates the transcription of snRNA genes through the recruitment of little elongation complex

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Title: MED26 regulates the transcription of snRNA genes through the recruitment of little elongation complex
Authors: Takahashi, Hidehisa Browse this author
Takigawa, Ichigaku Browse this author →KAKEN DB
Watanabe, Masashi Browse this author
Anwar, Delnur Browse this author
Shibata, Mio Browse this author
Tomomori-Sato, Chieri Browse this author
Sato, Shigeo Browse this author
Ranjan, Amol Browse this author
Seidel, Chris W. Browse this author
Tsukiyama, Tadasuke Browse this author →KAKEN DB
Mizushima, Wataru Browse this author
Hayashi, Masayasu Browse this author
Ohkawa, Yasuyuki Browse this author →KAKEN DB
Conaway, Joan W. Browse this author
Conaway, Ronald C. Browse this author
Hatakeyama, Shigetsugu Browse this author →KAKEN DB
Issue Date: Jan-2015
Publisher: Nature Publishing Group
Journal Title: Nature Communications
Volume: 6
Start Page: 5941
Publisher DOI: 10.1038/ncomms6941
Abstract: Regulation of transcription elongation by RNA polymerase II (Pol II) is a key regulatory step in gene transcription. Recently, the little elongation complex (LEC)-which contains the transcription elongation factor ELL/EAF-was found to be required for the transcription of Pol II-dependent small nuclear RNA (snRNA) genes. Here we show that the human Mediator subunit MED26 plays a role in the recruitment of LEC to a subset of snRNA genes through direct interaction of EAF and the N-terminal domain (NTD) of MED26. Loss of MED26 in cells decreases the occupancy of LEC at a subset of snRNA genes and results in a reduction in their transcription. Our results suggest that the MED26-NTD functions as a molecular switch in the exchange of TBP-associated factor 7 (TAF7) for LEC to facilitate the transition from initiation to elongation during transcription of a subset of snRNA genes.
Type: article (author version)
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 畠山 鎮次

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