Title: | MED26 regulates the transcription of snRNA genes through the recruitment of little elongation complex |
Authors: | Takahashi, Hidehisa Browse this author |
Takigawa, Ichigaku Browse this author →KAKEN DB |
Watanabe, Masashi Browse this author |
Anwar, Delnur Browse this author |
Shibata, Mio Browse this author |
Tomomori-Sato, Chieri Browse this author |
Sato, Shigeo Browse this author |
Ranjan, Amol Browse this author |
Seidel, Chris W. Browse this author |
Tsukiyama, Tadasuke Browse this author →KAKEN DB |
Mizushima, Wataru Browse this author |
Hayashi, Masayasu Browse this author |
Ohkawa, Yasuyuki Browse this author →KAKEN DB |
Conaway, Joan W. Browse this author |
Conaway, Ronald C. Browse this author |
Hatakeyama, Shigetsugu Browse this author →KAKEN DB |
Issue Date: | Jan-2015 |
Publisher: | Nature Publishing Group |
Journal Title: | Nature Communications |
Volume: | 6 |
Start Page: | 5941 |
Publisher DOI: | 10.1038/ncomms6941 |
Abstract: | Regulation of transcription elongation by RNA polymerase II (Pol II) is a key regulatory step in gene transcription. Recently, the little elongation complex (LEC)-which contains the transcription elongation factor ELL/EAF-was found to be required for the transcription of Pol II-dependent small nuclear RNA (snRNA) genes. Here we show that the human Mediator subunit MED26 plays a role in the recruitment of LEC to a subset of snRNA genes through direct interaction of EAF and the N-terminal domain (NTD) of MED26. Loss of MED26 in cells decreases the occupancy of LEC at a subset of snRNA genes and results in a reduction in their transcription. Our results suggest that the MED26-NTD functions as a molecular switch in the exchange of TBP-associated factor 7 (TAF7) for LEC to facilitate the transition from initiation to elongation during transcription of a subset of snRNA genes. |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/59508 |
Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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