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Type 1 collagen as a potential niche component for CD133-positive glioblastoma cells

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Title: Type 1 collagen as a potential niche component for CD133-positive glioblastoma cells
Authors: Motegi, Hiroaki Browse this author
Kamoshima, Yuuta Browse this author
Terasaka, Shunsuke Browse this author →KAKEN DB
Kobayashi, Hiroyuki Browse this author
Houkin, Kiyohiro Browse this author →KAKEN DB
Keywords: CD133
stem cell niche
stem cell
type 1 collagen
Issue Date: Aug-2014
Publisher: Wiley-Blackwell
Journal Title: Neuropathology
Volume: 34
Issue: 4
Start Page: 378
End Page: 385
Publisher DOI: 10.1111/neup.12117
PMID: 24673436
Abstract: Cancer stem cells are thought to be closely related to tumor progression and recurrence, making them attractive therapeutic targets. Stem cells of various tissues exist within niches maintaining their stemness. Glioblastoma stem cells (GSCs) are located at tumor capillaries and the perivascular niche, which are considered to have an important role in maintaining GSCs. There were some extracellular matrices (ECM) on the perivascular connective tissue, including type 1 collagen. We here evaluated whether type 1 collagen has a potential niche for GSCs. Imunohistochemical staining of type 1 collagen and CD133, one of the GSCs markers, on glioblastoma (GBM) tissues showed CD133-positive cells were located in immediate proximity to type 1 collagen around tumor vessels. We cultured human GBM cell lines, U87MG and GBM cells obtained from fresh surgical tissues, T472 and T555, with serum-containing medium (SCM) or serum-free medium with some growth factors (SFM) and in non-coated (Non-coat) or type 1 collagen-coated plates (Col). The RNA expression levels of CD133 and Nestin as stem cell markers in each condition were examined. The Col condition not only with SFM but SCM made GBM cells more enhanced in RNA expression of CD133, compared to Non-coat/SCM. Semi-quantitative measurement of CD133-positive cells by immunocytochemistry showed a statistically significant increase of CD133-positive cells in Col/SFM. In addition, T472 cell line cultured in the Col/SFM had capabilities of sphere formation and tumorigenesis. Type 1 collagen was found in the perivascular area and showed a possibility to maintain GSCs. These findings suggest that type 1 collagen could be one important niche component for CD133-positive GSCs and maintain GSCs in adherent culture.
Rights: This is the accepted version of the following article: Motegi, H., Kamoshima, Y., Terasaka, S., Kobayashi, H. and Houkin, K. (2014), Type 1 collagen as a potential niche component for CD133-positive glioblastoma cells. Neuropathology, 34: 378–385, which has been published in final form at
Type: article (author version)
Appears in Collections:北海道大学病院 (Hokkaido University Hospital) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 茂木 洋晃

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