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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
Peer-reviewed Journal Articles, etc >
Isolation of nine polymorphic microsatellite loci from the burying beetle, Nicrophorus quadripunctatus (Coleoptera: Silphidae)
| Title: | Isolation of nine polymorphic microsatellite loci from the burying beetle, Nicrophorus quadripunctatus (Coleoptera: Silphidae) |
| Authors: | Suzuki, Seizi Browse this author | | Yao, Izumi Browse this author →KAKEN DB |
| Keywords: | Carcass | | Habitat size | | Forest fragmentation | | Magnetic particles | | Genetic diversity |
| Issue Date: | Aug-2014 |
| Publisher: | Springer Japan |
| Journal Title: | Applied Entomology and Zoology |
| Volume: | 49 |
| Issue: | 3 |
| Start Page: | 493 |
| End Page: | 497 |
| Publisher DOI: | 10.1007/s13355-014-0269-8 |
| Abstract: | The burying beetle, Nicrophorus quadripunctatus Kraatz, is a common species in Japan, and its biparental care has been well studied. It exploits small vertebrate carrion as food for its young. Therefore, its reproductive success is restricted to large habitats in which carrion is abundant and available. Hence, the abundance of Nicrophorus species can be used as an indicator of forest fragmentation. Forests have recently become fragmented due to anthropogenic activities, and thus the population sizes of Nicrophorus species have decreased. To investigate the population genetic structure of N. quadripunctatus, we developed polymorphic microsatellite markers using magnetic particles. Nine microsatellites were polymorphic, with two to 12 alleles observed in the samples collected from our study sites, Matsunoyama and Nagaoka A and B. Deviation from the Hardy-Weinberg equilibrium was detected at five to seven loci in each population examined, which is indicative of significant heterozygous deficiencies. Higher genetic diversity was found in the Matsunoyama and Nagaoka B populations, the collection plots of which were covered by continuous woodlands, compared to Nagaoka A. The isolated microsatellite markers will be used to determine the genetic structures of the fragmented populations of N. quadripunctatus. |
| Rights: | The final publication is available at www.springerlink.com |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/59754 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 八尾 泉
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