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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Life Science / Faculty of Advanced Life Science >
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Structural and functional analysis of the Rpf2-Rrs1 complex in ribosome biogenesis
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| Title: | Structural and functional analysis of the Rpf2-Rrs1 complex in ribosome biogenesis |
| Authors: | Asano, Nozomi Browse this author | | Kato, Koji Browse this author | | Nakamura, Akiyoshi Browse this author | | Komoda, Keisuke Browse this author | | Tanaka, Isao Browse this author | | Yao, Min Browse this author →KAKEN DB |
| Issue Date: | 19-May-2015 |
| Publisher: | Oxford University Press |
| Journal Title: | Nucleic acids research |
| Volume: | 43 |
| Issue: | 9 |
| Start Page: | 4746 |
| End Page: | 4757 |
| Publisher DOI: | 10.1093/nar/gkv305 |
| PMID: | 25855814 |
| Abstract: | Proteins Rpf2 and Rrs1 are required for 60S ribosomal subunit maturation. These proteins are necessary for the recruitment of three ribosomal components (5S ribosomal RNA [rRNA], RpL5 and RpL11) to the 90S ribosome precursor and subsequent 27SB pre-rRNA processing. Here we present the crystal structure of the Aspergillus nidulans (An) Rpf2-Rrs1 core complex. The core complex contains the tightly interlocked N-terminal domains of Rpf2 and Rrs1. The Rpf2 N-terminal domain includes a Brix domain characterized by similar N-and C-terminal architecture. The long alpha-helix of Rrs1 joins the C-terminal half of the Brix domain as if it were part of a single molecule. The conserved proline-rich linker connecting the N- and C-terminal domains of Rrs1 wrap around the side of Rpf2 and anchor the C-terminal domain of Rrs1 to a specific site on Rpf2. In addition, gel shift analysis revealed that the Rpf2-Rrs1 complex binds directly to 5S rRNA. Further analysis of Rpf2-Rrs1 mutants demonstrated that Saccha-romyces cerevisiae Rpf2 R236 (corresponds to R238 of AnRpf2) plays a significant role in this binding. Based on these studies and previous reports, we have proposed a model for ribosomal component recruitment to the 90S ribosome precursor. |
| Rights: | http://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/59772 |
| Appears in Collections: | 生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 姚 閔
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