| Title: | Comparative analysis of different enzyme immunoassays for assessment of phosphatidylserine-dependent antiprothrombin antibodies |
| Authors: | Amengual, Olga Browse this author →KAKEN DB |
| Horita, Tetsuya Browse this author →KAKEN DB |
| Binder, Walter Browse this author |
| Norman, Gary L. Browse this author |
| Shums, Zakera Browse this author |
| Kato, Masaru Browse this author →KAKEN DB |
| Otomo, Kotaro Browse this author |
| Fujieda, Yuichiro Browse this author |
| Oku, Kenji Browse this author |
| Bohgaki, Toshiyuki Browse this author |
| Yasuda, Shinsuke Browse this author →KAKEN DB |
| Atsumi, Tatsuya Browse this author →KAKEN DB |
| Keywords: | Antiphospholipid syndrome |
| Antiphospholipid antibodies |
| Thrombosis |
| Lupus anticoagulant |
| Issue Date: | Sep-2014 |
| Publisher: | Springer |
| Journal Title: | Rheumatology international |
| Volume: | 34 |
| Issue: | 9 |
| Start Page: | 1225 |
| End Page: | 1230 |
| Publisher DOI: | 10.1007/s00296-014-2951-0 |
| PMID: | 24497039 |
| Abstract: | Phosphatidylserine-dependent antiprothrombin antibodies (aPS/PT) were strongly correlated with the presence of lupus anticoagulant showing a high specificity for the diagnosis of antiphospholipid syndrome. However, the main criticism for the clinical applicability of aPS/PT testing is the lack of reproducibility of the results among laboratories. In this study, we measured IgG and IgM aPS/PT using our original in-house enzyme-linked immunosorbent assays (ELISA) and commercial ELISA kits to assess the assay performance and to evaluate the accuracy of aPS/PT results. The study included 111 plasma samples collected from patients and stored at our laboratory for aPS/PT assessment. Sixty-one samples were tested for IgG aPS/PT using two assays: (1) aPS/PT in-house ELISA and (2) QUANTA Lite (TM) aPS/PT IgG ELISA kit (INOVA Diagnostics, Inc., USA). Fifty samples were evaluated for IgM aPS/PT using two assays: (1) aPS/PT in-house ELISA and (2) QUANTA Lite (TM) aPS/PT IgM ELISA kit (INOVA Diagnostics). Ninety-eight percent of samples yielded concordant results for IgG aPS/PT and 82 % for IgM aPS/PT. There was an excellent agreement between the IgG aPS/PT assays (Cohen kappa = 0.962) and moderate agreement between the IgM aPS/PT assays (kappa = 0.597). Statistically significant correlations in the aPS/PT results were obtained from both IgG and IgM aPS/PT assays (r = 0.749, r = 0.622, p < 0.001, respectively). In conclusion, IgG and IgM detection by ELISA is accurate. The performance of aPS/PT is reliable, and concordant results can be obtained using different ELISA methods. |
| Rights: | The final publication is available at link.springer.com |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/59853 |
| Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
