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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Dental Medicine / Faculty of Dental Medicine >
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Ectopic Bone Induction by BMP-loaded Collagen Scaffold and Bone Marrow Stromal Cell Sheet
| Title: | Ectopic Bone Induction by BMP-loaded Collagen Scaffold and Bone Marrow Stromal Cell Sheet |
| Authors: | INOUE, Kana1 Browse this author | | MIYAJI, Hirofumi2 Browse this author →KAKEN DB | | SUGAYA, Tsutomu3 Browse this author →KAKEN DB | | KAWANAMI, Masamitsu4 Browse this author →KAKEN DB |
| Authors(alt): | INOUE, Kana1 | | MIYAJI, Hirofumi2 | | SUGAYA, Tsutomu3 | | KAWANAMI, Masamitsu4 |
| Keywords: | bone tissue engineering | | bone marrow stromal cell | | BMP-2 | | cell sheet | | collagen sponge scaffold |
| Issue Date: | 30-Sep-2010 |
| Publisher: | Japanese Association of Regenerative Dentistry | | 日本再生歯科医学会 |
| Journal Title: | Journal of Oral Tissue Engineering |
| Volume: | 8 |
| Issue: | 1 |
| Start Page: | 19 |
| End Page: | 29 |
| Publisher DOI: | 10.11223/jarde.8.19 |
| Abstract: | We combined the use of bone morphogenetic protein-2(BMP-2)-loaded collagen scaffold and cultured bone marrow stromal cell (BMSC) sheet in order for developing new bone tissue engineering.Rat BMSC sheets were generated in the presence of dexamethasone, β-glycerophosphate and ascorbic acid. Four types of the grafts were implanted in the intermuscular region of rat femurs: control group, collagen sponge; cell group, BMSC sheet in collagen sponge; BMP group, BMP-2-loaded collagen sponge; and BMP-cell group, BMSC sheet in BMP-2-loaded collagen sponge. Four weeks after surgery, histological and histomorphometric analysis were performed.The BMP-cell group demonstrated abundant new bone formation compared to all the other groups (p<0.05) and marked resorption of the implanted collagen when compared to the control and cell groups (p<0.05).The combination of BMP-2-loaded collagen sponge and BMSC sheet appeared to successfully promote an osteoinduction. |
| Rights: | 日本再生歯科医学会(利用は著作権の範囲内に限られます) |
| Type: | article |
| URI: | http://hdl.handle.net/2115/59875 |
| Appears in Collections: | 歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 宮治 裕史
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