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Ectopic Bone Induction by BMP-loaded Collagen Scaffold and Bone Marrow Stromal Cell Sheet

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Title: Ectopic Bone Induction by BMP-loaded Collagen Scaffold and Bone Marrow Stromal Cell Sheet
Authors: INOUE, Kana1 Browse this author
MIYAJI, Hirofumi2 Browse this author →KAKEN DB
SUGAYA, Tsutomu3 Browse this author →KAKEN DB
KAWANAMI, Masamitsu4 Browse this author →KAKEN DB
Authors(alt): INOUE, Kana1
MIYAJI, Hirofumi2
SUGAYA, Tsutomu3
KAWANAMI, Masamitsu4
Keywords: bone tissue engineering
bone marrow stromal cell
cell sheet
collagen sponge scaffold
Issue Date: 30-Sep-2010
Publisher: Japanese Association of Regenerative Dentistry
Journal Title: Journal of Oral Tissue Engineering
Volume: 8
Issue: 1
Start Page: 19
End Page: 29
Publisher DOI: 10.11223/jarde.8.19
Abstract: We combined the use of bone morphogenetic protein-2(BMP-2)-loaded collagen scaffold and cultured bone marrow stromal cell (BMSC) sheet in order for developing new bone tissue engineering.Rat BMSC sheets were generated in the presence of dexamethasone, β-glycerophosphate and ascorbic acid. Four types of the grafts were implanted in the intermuscular region of rat femurs: control group, collagen sponge; cell group, BMSC sheet in collagen sponge; BMP group, BMP-2-loaded collagen sponge; and BMP-cell group, BMSC sheet in BMP-2-loaded collagen sponge. Four weeks after surgery, histological and histomorphometric analysis were performed.The BMP-cell group demonstrated abundant new bone formation compared to all the other groups (p<0.05) and marked resorption of the implanted collagen when compared to the control and cell groups (p<0.05).The combination of BMP-2-loaded collagen sponge and BMSC sheet appeared to successfully promote an osteoinduction.
Rights: 日本再生歯科医学会(利用は著作権の範囲内に限られます)
Type: article
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 宮治 裕史

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