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Volume 63 Number 3 >

Diagnostic application of recombinant equine merozoite surface antigen-1 in elisa for detection of Theileria equi specific antibodies

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Title: Diagnostic application of recombinant equine merozoite surface antigen-1 in elisa for detection of Theileria equi specific antibodies
Authors: Kumar, Sandeep Browse this author
Rakha, Naresh K. Browse this author
Goyal, Liza Browse this author
Goel, Parveen Browse this author
Kumar, Rajender Browse this author
Kumar, Ashok Browse this author
Kumar, Sanjay Browse this author
Keywords: Theileria equi
Babesia equi
Issue Date: Aug-2015
Publisher: Graduate School of Veterinary Medicine, Hokkaido University
Journal Title: Japanese Journal of Veterinary Research
Volume: 63
Issue: 3
Start Page: 129
End Page: 137
Abstract: Theileria equi merozoite surface antigens have been an important candidate for development of diagnostics. We developed ELISA based on EMA-1 recombinant antigen, so as to widen our diagnostic confidence in detection of antibodies against T. equi in serosurveillance studies. The 547 bp EMA-1 gene fragment encoding high hydrophilic antigenic region was expressed with glutathione-S-transferase tag in prokaryotic system and purified protein (43 kDa) was used for development of ELISA (EMA-1t/ELISA). The EMA-1t/ELISA clearly differentiated T. equi-infected from Babesia caballi-infected horse sera or normal horse sera. The results of the study were validated with previously developed EMA-2ELISA on serum samples of known T. equi infection status and a very high correlation (0.93) was recorded between the relative percent positivity (RPP) values. Further diagnostic sensitivity of EMA-1t/ELISA was 0.92 while specificity was 1.0, indicating its suitability for sero-epidemiological studies. This assay was applied on serum samples (n = 240) collected from field horses in northern part of India. High sero-prevalence of T. equi antibodies were diagnosed in serum samples collected from Haryana state (74%) and Uttarakhand state (36.31%). Results of this study suggested that the 43 kDa EMA-1 expressed protein could be a reliable immunodiagnostic antigen in ELISA for T. equi sero-prevalence studies.
Type: bulletin (article)
Appears in Collections:Japanese Journal of Veterinary Research > Volume 63 Number 3

Submitter: 獣医学部図書室

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