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Volume 63 Number 3 >

Nfkbiz regulates the proliferation and differentiation of keratinocytes

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Title: Nfkbiz regulates the proliferation and differentiation of keratinocytes
Authors: Ishiguro-Oonuma, Toshina Browse this author →KAKEN DB
Ochiai, Kazuhiko Browse this author →KAKEN DB
Hashizume, Kazuyoshi Browse this author →KAKEN DB
Iwanaga, Toshihiko Browse this author →KAKEN DB
Morimatsu, Masami Browse this author →KAKEN DB
Keywords: IκBζ
Issue Date: Aug-2015
Publisher: Graduate School of Veterinary Medicine, Hokkaido University
Journal Title: Japanese Journal of Veterinary Research
Volume: 63
Issue: 3
Start Page: 107
End Page: 114
Abstract: Nuclear factor of kappa light polypeptide gene enhancer in B cells (NF-κB) inhibitor zeta (Nfkbiz) is a nuclear inhibitor of NF-κB (IκB) protein that is also termed as molecule possessing ankyrin repeats induced by lipopolysaccharide, interleukin-1-inducible nuclear ankyrin repeat protein, or IκBζ. We found previously that disrupting the Nfkbiz gene resulted in atopic dermatitis-like lesions in mice, suggesting an important role for Nfkbiz in the skin. In this study, we examined the cellular function of Nfkbiz in keratinocytes. Immunohistochemical analyses for Ki-67 revealed that Nfkbiz-/- keratinocytes were hypoproliferative. In skin from Nfkbiz-/- mice, the expression of the keratinocyte differentiation markers K10 and filaggrin were reduced, although that of K14 was unchanged. The terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling assay revealed that the frequency of apoptosis was comparable between control and Nfkbiz-/- keratinocytes. Interestingly, the subcellular localization of the NF-κB subunits and the transcriptional activity of NF-κB were not changed in Nfkbiz-/- keratinocytes. These findings indicate a novel possible role of Nfkbiz in controlling the proliferation and differentiation of epidermal keratinocytes through NF-κB-independent mechanisms.
Type: bulletin (article)
Appears in Collections:Japanese Journal of Veterinary Research > Volume 63 Number 3

Submitter: 獣医学部図書室

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