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SWI/SNF chromatin-remodeling complexes function in noncoding RNA-dependent assembly of nuclear bodies
| Title: | SWI/SNF chromatin-remodeling complexes function in noncoding RNA-dependent assembly of nuclear bodies |
| Authors: | Kawaguchi, Tetsuya Browse this author | | Tanigawa, Akie Browse this author | | Naganuma, Takao Browse this author | | Ohkawa, Yasuyuki Browse this author | | Souquere, Sylvie Browse this author | | Pierron, Gerard Browse this author | | Hirose, Tetsuro Browse this author |
| Keywords: | chromatin-remodeling complex | | long noncoding RNA | | nuclear bodies | | ribonucleoprotein assembly |
| Issue Date: | 8-Apr-2015 |
| Publisher: | The National Academy of Sciences |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 112 |
| Issue: | 14 |
| Start Page: | 4304 |
| End Page: | 4309 |
| Publisher DOI: | 10.1073/pnas.1423819112 |
| Abstract: | Paraspeckles are subnuclear structures that form around nuclear paraspeckle assembly transcript 1 (NEAT1) long noncoding RNA (lncRNA). Recently, paraspeckles were shown to be functional nuclear bodies involved in stress responses and the development of specific organs. Paraspeckle formation is initiated by transcription of the NEAT1 chromosomal locus and proceeds in conjunction with NEAT1 lncRNA biogenesis and a subsequent assembly step involving >40 paraspeckle proteins (PSPs). In this study, subunits of SWItch/Sucrose NonFermentable (SWI/SNF) chromatin-remodeling complexes were identified as paraspeckle components that interact with PSPs and NEAT1 lncRNA. EM observations revealed that SWI/SNF complexes were enriched in paraspeckle subdomains depleted of chromatin. Knockdown of SWI/SNF components resulted in paraspeckle disintegration, but mutation of the ATPase domain of the catalytic subunit BRG1 did not affect paraspeckle integrity, indicating that the essential role of SWI/SNF complexes in paraspeckle formation does not require their canonical activity. Knockdown of SWI/SNF complexes barely affected the levels of known essential paraspeckle components, but markedly diminished the interactions between essential PSPs, suggesting that SWI/SNF complexes facilitate organization of the PSP interaction network required for intact paraspeckle assembly. The interactions between SWI/SNF components and essential PSPs were maintained in NEAT1-depleted cells, suggesting that SWI/SNF complexes not only facilitate interactions between PSPs, but also recruit PSPs during paraspeckle assembly. SWI/SNF complexes were also required for Satellite III lncRNA-dependent formation of nuclear stress bodies under heat-shock conditions. Our data suggest the existence of a common mechanism underlying the formation of lncRNA-dependent nuclear body architectures in mammalian cells. |
| Description: | This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10.1073/pnas.1423819112/-/DCSupplemental. |
| Description URI: | http://www.pnas.org/lookup/suppl/doi:10.1073/pnas.1423819112/-/DCSupplemental |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/59917 |
| Appears in Collections: | 遺伝子病制御研究所 (Institute for Genetic Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 廣瀬 哲郎
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