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Hydrogen/deuterium exchange of cross-linkable alpha-amino acid derivatives in deuterated triflic acid
| Title: | Hydrogen/deuterium exchange of cross-linkable alpha-amino acid derivatives in deuterated triflic acid |
| Authors: | Wang, Lei Browse this author | | Murai, Yuta Browse this author | | Yoshida, Takuma Browse this author | | Okamoto, Masashi Browse this author | | Masuda, Katsuyoshi Browse this author →KAKEN DB | | Sakihama, Yasuko Browse this author →KAKEN DB | | Hashidoko, Yasuyuki Browse this author →KAKEN DB | | Hatanaka, Yasumaru Browse this author →KAKEN DB | | Hashimoto, Makoto Browse this author →KAKEN DB |
| Keywords: | Hydrogen/deuterium exchange | | aromatic alpha-amino acid | | cross-link | | photophore | | triflic acid |
| Issue Date: | Jul-2014 |
| Publisher: | Taylor & Francis |
| Journal Title: | Bioscience Biotechnology and Biochemistry |
| Volume: | 78 |
| Issue: | 7 |
| Start Page: | 1129 |
| End Page: | 1134 |
| Publisher DOI: | 10.1080/09168451.2014.917267 |
| PMID: | 25229846 |
| Abstract: | In this paper we report here a hydrogen/deuterium exchange (H/D exchange) of cross-linkable alpha-amino acid derivatives with deuterated trifluoromethanesulfonic acid (TfOD). H/D exchange with TfOD was easily applied to o-catechol containing phenylalanine (DOPA) within an hour. A partial H/D exchange was observed for trifluoromethyldiazirinyl (TFMD) phenylalanine derivatives. N-Acetyl-protected natural aromatic alpha-amino acids (Tyr and Trp) were more effective in H/D exchange than unprotected ones. The N-acetylated TFMD phenylalanine derivative afforded slightly higher H/D exchange than unprotected derivatives. An effective post-deuteration method for cross-linkable alpha-amino acid derivatives will be useful for the analysis of biological functions of bioactive peptides and proteins by mass spectrometry. |
| Rights: | This is an Accepted Manuscript of an article published by Taylor & Francis in [JOURNAL TITLE] on [date of publication], available online: http://www.tandfonline.com/DOI:10.1080/09168451.2014.917267. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/60049 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 橋本 誠
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