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Visualization of the entire differentiation process of murine M cells : suppression of their maturation in cecal patches
| Title: | Visualization of the entire differentiation process of murine M cells : suppression of their maturation in cecal patches |
| Authors: | Kimura, Shunsuke Browse this author →KAKEN DB | | Yamakami-Kimura, Megumi Browse this author | | Obata, Yuuki Browse this author | | Hase, Koji Browse this author | | Kitamura, Hiroshi Browse this author →KAKEN DB | | Ohno, Hiroshi Browse this author →KAKEN DB | | Iwanaga, Toshihiko Browse this author →KAKEN DB |
| Issue Date: | May-2015 |
| Publisher: | Nature Publishing Group |
| Journal Title: | Mucosal immunology |
| Volume: | 8 |
| Issue: | 3 |
| Start Page: | 650 |
| End Page: | 660 |
| Publisher DOI: | 10.1038/mi.2014.99 |
| PMID: | 25336168 |
| Abstract: | The microfold (M) cell residing in the follicle-associated epithelium is a specialized epithelial cell that initiates mucosal immune responses by sampling lumina! antigens. The differentiation process of M cells remains unclear due to limitations of analytical methods. Here we found that M cells were classified into two functionally different subtypes based on the expression of Glycoprotein 2 (GP2) by newly developed image cytometric analysis. GP2-high M cells actively took up luminal microbeads, whereas GP2-negative or low cells scarcely ingested them, even though both subsets equally expressed the other M-cell signature genes, suggesting that GP2-high M cells represent functionally mature M cells. Further, the GP2-high mature M cells were abundant in Peyer's patch but sparse in the cecal patch: this was most likely due to a decrease in the nuclear translocation of RelB, a downstream transcription factor for the receptor activator of nuclear factor-kappa B signaling. Given that murine cecum contains a protrusion of beneficial commensals, the restriction of M-cell activity might contribute to preventing the onset of any excessive immune response to the commensals through decelerating the M-cell-dependent uptake of microorganisms. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/60152 |
| Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 木村 俊介
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