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Preimplantation death of xenomitochondrial mouse embryo harbouring bovine mitochondria

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Title: Preimplantation death of xenomitochondrial mouse embryo harbouring bovine mitochondria
Authors: Kawahara, Manabu Browse this author →KAKEN DB
Koyama, Shiori Browse this author
Iimura, Satomi Browse this author
Yamazaki, Wataru Browse this author
Tanaka, Aiko Browse this author
Kohri, Nanami Browse this author
Sasaki, Keisuke Browse this author
Takahashi, Masashi Browse this author →KAKEN DB
Issue Date: 30-Sep-2015
Publisher: Nature Publishing Group
Journal Title: Scientific reports
Volume: 5
Start Page: 14512
Publisher DOI: 10.1038/srep14512
Abstract: Mitochondria, cellular organelles playing essential roles in eukaryotic cell metabolism, are thought to have evolved from bacteria. The organization of mtDNA is remarkably uniform across species, reflecting its vital and conserved role in oxidative phosphorylation (OXPHOS). Our objectives were to evaluate the compatibility of xenogeneic mitochondria in the development of preimplantation embryos in mammals. Mouse embryos harbouring bovine mitochondria (mtB-M embryos) were prepared by the cell-fusion technique employing the haemagglutinating virus of Japan (HVJ). The mtB-M embryos showed developmental delay at embryonic days (E) 3.5 after insemination. Furthermore, none of the mtB-M embryos could implant into the maternal uterus after embryo transfer, whereas control mouse embryos into which mitochondria from another mouse had been transferred developed as well as did non-manipulated embryos. When we performed quantitative PCR (qPCR) of mouse and bovine ND5, we found that the mtB-M embryos contained 8.3% of bovine mitochondria at the blastocyst stage. Thus, contamination with mitochondria from another species induces embryonic lethality prior to implantation into the maternal uterus. The heteroplasmic state of these xenogeneic mitochondria could have detrimental effects on preimplantation development, leading to preservation of species-specific mitochondrial integrity in mammals.
Type: article
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 川原 学

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