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A Single Amino Acid in the M1 Protein Responsible for the Different Pathogenic Potentials of H5N1 Highly Pathogenic Avian Influenza Virus Strains

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Title: A Single Amino Acid in the M1 Protein Responsible for the Different Pathogenic Potentials of H5N1 Highly Pathogenic Avian Influenza Virus Strains
Authors: Nao, Naganori Browse this author
Kajihara, Masahiro Browse this author →KAKEN DB
Manzoor, Rashid Browse this author
Maruyama, Junki Browse this author
Yoshida, Reiko Browse this author →KAKEN DB
Muramatsu, Mieko Browse this author
Miyamoto, Hiroko Browse this author
Igarashi, Manabu Browse this author →KAKEN DB
Eguchi, Nao Browse this author
Sato, Masahiro Browse this author
Kondoh, Tatsunari Browse this author
Okamatsu, Masatoshi Browse this author →KAKEN DB
Sakoda, Yoshihiro Browse this author →KAKEN DB
Kida, Hiroshi Browse this author →KAKEN DB
Takada, Ayato Browse this author →KAKEN DB
Issue Date: 14-Sep-2015
Publisher: Public Library of Science
Journal Title: PLoS ONE
Volume: 10
Issue: 9
Start Page: e0137989
Publisher DOI: 10.1371/journal.pone.0137989
Abstract: Two highly pathogenic avian influenza virus strains, A/duck/Hokkaido/WZ83/2010 ( H5N1) (WZ83) and A/duck/Hokkaido/WZ101/2010 (H5N1) (WZ101), which were isolated from wild ducks in Japan, were found to be genetically similar, with only two amino acid differences in their M1 and PB1 proteins at positions 43 and 317, respectively. We found that both WZ83 and WZ101 caused lethal infection in chickens but WZ101 killed them more rapidly than WZ83. Interestingly, ducks experimentally infected with WZ83 showed no or only mild clinical symptoms, whereas WZ101 was highly lethal. We then generated reassortants between these viruses and found that exchange of the M gene segment completely switched the pathogenic phenotype in both chickens and ducks, indicating that the difference in the pathogenicity for these avian species between WZ83 and WZ101 was determined by only a single amino acid in the M1 protein. It was also found that WZ101 showed higher pathogenicity than WZ83 in mice and that WZ83, whose M gene was replaced with that of WZ101, showed higher pathogenicity than wild-type WZ83, although this reassortant virus was not fully pathogenic compared to wild-type WZ101. These results suggest that the amino acid at position 43 of the M1 protein is one of the factors contributing to the pathogenicity of H5N1 highly pathogenic avian influenza viruses in both avian and mammalian hosts.
Type: article
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 高田 礼人

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