| Title: | A Single Amino Acid in the M1 Protein Responsible for the Different Pathogenic Potentials of H5N1 Highly Pathogenic Avian Influenza Virus Strains |
| Authors: | Nao, Naganori Browse this author |
| Kajihara, Masahiro Browse this author →KAKEN DB |
| Manzoor, Rashid Browse this author |
| Maruyama, Junki Browse this author |
| Yoshida, Reiko Browse this author →KAKEN DB |
| Muramatsu, Mieko Browse this author |
| Miyamoto, Hiroko Browse this author |
| Igarashi, Manabu Browse this author →KAKEN DB |
| Eguchi, Nao Browse this author |
| Sato, Masahiro Browse this author |
| Kondoh, Tatsunari Browse this author |
| Okamatsu, Masatoshi Browse this author →KAKEN DB |
| Sakoda, Yoshihiro Browse this author →KAKEN DB |
| Kida, Hiroshi Browse this author →KAKEN DB |
| Takada, Ayato Browse this author →KAKEN DB |
| Issue Date: | 14-Sep-2015 |
| Publisher: | Public Library of Science |
| Journal Title: | PLoS ONE |
| Volume: | 10 |
| Issue: | 9 |
| Start Page: | e0137989 |
| Publisher DOI: | 10.1371/journal.pone.0137989 |
| Abstract: | Two highly pathogenic avian influenza virus strains, A/duck/Hokkaido/WZ83/2010 ( H5N1) (WZ83) and A/duck/Hokkaido/WZ101/2010 (H5N1) (WZ101), which were isolated from wild ducks in Japan, were found to be genetically similar, with only two amino acid differences in their M1 and PB1 proteins at positions 43 and 317, respectively. We found that both WZ83 and WZ101 caused lethal infection in chickens but WZ101 killed them more rapidly than WZ83. Interestingly, ducks experimentally infected with WZ83 showed no or only mild clinical symptoms, whereas WZ101 was highly lethal. We then generated reassortants between these viruses and found that exchange of the M gene segment completely switched the pathogenic phenotype in both chickens and ducks, indicating that the difference in the pathogenicity for these avian species between WZ83 and WZ101 was determined by only a single amino acid in the M1 protein. It was also found that WZ101 showed higher pathogenicity than WZ83 in mice and that WZ83, whose M gene was replaced with that of WZ101, showed higher pathogenicity than wild-type WZ83, although this reassortant virus was not fully pathogenic compared to wild-type WZ101. These results suggest that the amino acid at position 43 of the M1 protein is one of the factors contributing to the pathogenicity of H5N1 highly pathogenic avian influenza viruses in both avian and mammalian hosts. |
| Rights: | http://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/60254 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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