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Hertwig's epithelial root sheath cell behavior during initial acellular cementogenesis in rat molars

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Title: Hertwig's epithelial root sheath cell behavior during initial acellular cementogenesis in rat molars
Authors: Yamamoto, Tsuneyuki Browse this author →KAKEN DB
Yamamoto, Tomomaya Browse this author
Yamada, Tamaki Browse this author
Hasegawa, Tomoka Browse this author
Hongo, Hiromi Browse this author
Oda, Kimimitsu Browse this author
Amizuka, Norio Browse this author →KAKEN DB
Keywords: Hertwig's epithelial root sheath
Epithelial-mesenchymal transition
Acellular cementum
Rat molars
Issue Date: Nov-2014
Publisher: Springer
Journal Title: Histochemistry and cell biology
Volume: 142
Issue: 5
Start Page: 489
End Page: 496
Publisher DOI: 10.1007/s00418-014-1230-1
PMID: 24859538
Abstract: This study was designed to examine developing acellular cementum in rat molars by immunohistochemistry, to elucidate (1) how Hertwig's epithelial root sheath disintegrates and (2) whether epithelial sheath cells transform into cementoblasts through epithelial-mesenchymal transition (EMT). Initial acellular cementogenesis was divided into three developmental stages, which can be seen in three different portions of the root: portion 1, where the epithelial sheath is intact; portion 2, where the epithelial sheath becomes fragmented; and portion 3, where acellular cementogenesis begins. Antibodies against three kinds of matrix proteinases, which degrade epithelial sheath-maintaining factors, including basement membrane and desmosomes, were used to investigate proteolytic activity of the epithelial sheath. Tissue non-specific alkaline phosphatase (TNALP) and keratin were used to investigate EMT. Epithelial sheath cells showed immunoreactivity for all three enzymes at fragmentation, which suggests that epithelial sheath disintegration is enzymatically mediated. Dental follicle cells and cementoblasts showed intense immunoreactivity for TNALP, and from portion 1 through to 3, the reaction extended from the alveolar bone-related zone to the root-related zone. Cells possessing keratin/TNALP double immunoreactivity were virtually absent. Keratin-positive epithelial sheath cells showed negligible immunoreactivity for TNALP, and epithelial cells did not appear to migrate to the dental follicle. Together, these findings suggest that a transition phenotype between epithelial cells and cementoblasts does not exist in the developing dental follicle and hence that epithelial sheath cells do not undergo EMT during initial acellular cementogenesis. In brief, this study supports the notion that cementoblasts derive from the dental follicle.
Rights: The final publication is available at
Type: article (author version)
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 山本 恒之

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