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Hokkaido University Collection of Scholarly and Academic Papers >
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Hertwig's epithelial root sheath cell behavior during initial acellular cementogenesis in rat molars
| Title: | Hertwig's epithelial root sheath cell behavior during initial acellular cementogenesis in rat molars |
| Authors: | Yamamoto, Tsuneyuki Browse this author →KAKEN DB | | Yamamoto, Tomomaya Browse this author | | Yamada, Tamaki Browse this author | | Hasegawa, Tomoka Browse this author | | Hongo, Hiromi Browse this author | | Oda, Kimimitsu Browse this author | | Amizuka, Norio Browse this author →KAKEN DB |
| Keywords: | Hertwig's epithelial root sheath | | Cementoblasts | | Epithelial-mesenchymal transition | | Acellular cementum | | Rat molars |
| Issue Date: | Nov-2014 |
| Publisher: | Springer |
| Journal Title: | Histochemistry and cell biology |
| Volume: | 142 |
| Issue: | 5 |
| Start Page: | 489 |
| End Page: | 496 |
| Publisher DOI: | 10.1007/s00418-014-1230-1 |
| PMID: | 24859538 |
| Abstract: | This study was designed to examine developing acellular cementum in rat molars by immunohistochemistry, to elucidate (1) how Hertwig's epithelial root sheath disintegrates and (2) whether epithelial sheath cells transform into cementoblasts through epithelial-mesenchymal transition (EMT). Initial acellular cementogenesis was divided into three developmental stages, which can be seen in three different portions of the root: portion 1, where the epithelial sheath is intact; portion 2, where the epithelial sheath becomes fragmented; and portion 3, where acellular cementogenesis begins. Antibodies against three kinds of matrix proteinases, which degrade epithelial sheath-maintaining factors, including basement membrane and desmosomes, were used to investigate proteolytic activity of the epithelial sheath. Tissue non-specific alkaline phosphatase (TNALP) and keratin were used to investigate EMT. Epithelial sheath cells showed immunoreactivity for all three enzymes at fragmentation, which suggests that epithelial sheath disintegration is enzymatically mediated. Dental follicle cells and cementoblasts showed intense immunoreactivity for TNALP, and from portion 1 through to 3, the reaction extended from the alveolar bone-related zone to the root-related zone. Cells possessing keratin/TNALP double immunoreactivity were virtually absent. Keratin-positive epithelial sheath cells showed negligible immunoreactivity for TNALP, and epithelial cells did not appear to migrate to the dental follicle. Together, these findings suggest that a transition phenotype between epithelial cells and cementoblasts does not exist in the developing dental follicle and hence that epithelial sheath cells do not undergo EMT during initial acellular cementogenesis. In brief, this study supports the notion that cementoblasts derive from the dental follicle. |
| Rights: | The final publication is available at link.springer.com |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/60279 |
| Appears in Collections: | 歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 山本 恒之
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