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The cell competition-based high-throughput screening identifies small compounds that promote the elimination of RasV12-transformed cells from epithelia
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| Title: | The cell competition-based high-throughput screening identifies small compounds that promote the elimination of RasV12-transformed cells from epithelia |
| Authors: | Yamauchi, Hajime Browse this author | | Matsumaru, Takanori Browse this author | | Morita, Tomoko Browse this author | | Ishikawa, Susumu Browse this author | | Maenaka, Katsumi Browse this author →KAKEN DB | | Takigawa, Ichigaku Browse this author →KAKEN DB | | Semba, Kentaro Browse this author →KAKEN DB | | Kon, Shunsuke Browse this author →KAKEN DB | | Fujita, Yasuyuki Browse this author →KAKEN DB |
| Issue Date: | 21-Oct-2015 |
| Publisher: | Nature Publishing Group |
| Journal Title: | Scientific reports |
| Volume: | 5 |
| Start Page: | 15336 |
| Publisher DOI: | 10.1038/srep15336 |
| Abstract: | Recent studies have revealed that cell competition can occur between normal and transformed epithelial cells; normal epithelial cells recognize the presence of the neighboring transformed cells and actively eliminate them from epithelial tissues. Here, we have established a brand-new high-throughput screening platform that targets cell competition. By using this platform, we have identified Rebeccamycin as a hit compound that specifically promotes elimination of RasV12-transformed cells from the epithelium, though after longer treatment it shows substantial cytotoxic effect against normal epithelial cells. Among several Rebeccamycin-derivative compounds, we have found that VC1-8 has least cytotoxicity against normal cells but shows the comparable effect on the elimination of transformed cells. This cell competition-promoting activity of VC1-8 is observed both in vitro and ex vivo. These data demonstrate that the cell competition-based screening is a promising tool for the establishment of a novel type of cancer preventive medicine. |
| Rights: | http://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/60284 |
| Appears in Collections: | 遺伝子病制御研究所 (Institute for Genetic Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 藤田 恭之
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