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The cell competition-based high-throughput screening identifies small compounds that promote the elimination of RasV12-transformed cells from epithelia

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Title: The cell competition-based high-throughput screening identifies small compounds that promote the elimination of RasV12-transformed cells from epithelia
Authors: Yamauchi, Hajime Browse this author
Matsumaru, Takanori Browse this author
Morita, Tomoko Browse this author
Ishikawa, Susumu Browse this author
Maenaka, Katsumi Browse this author →KAKEN DB
Takigawa, Ichigaku Browse this author →KAKEN DB
Semba, Kentaro Browse this author →KAKEN DB
Kon, Shunsuke Browse this author →KAKEN DB
Fujita, Yasuyuki Browse this author →KAKEN DB
Issue Date: 21-Oct-2015
Publisher: Nature Publishing Group
Journal Title: Scientific reports
Volume: 5
Start Page: 15336
Publisher DOI: 10.1038/srep15336
Abstract: Recent studies have revealed that cell competition can occur between normal and transformed epithelial cells; normal epithelial cells recognize the presence of the neighboring transformed cells and actively eliminate them from epithelial tissues. Here, we have established a brand-new high-throughput screening platform that targets cell competition. By using this platform, we have identified Rebeccamycin as a hit compound that specifically promotes elimination of RasV12-transformed cells from the epithelium, though after longer treatment it shows substantial cytotoxic effect against normal epithelial cells. Among several Rebeccamycin-derivative compounds, we have found that VC1-8 has least cytotoxicity against normal cells but shows the comparable effect on the elimination of transformed cells. This cell competition-promoting activity of VC1-8 is observed both in vitro and ex vivo. These data demonstrate that the cell competition-based screening is a promising tool for the establishment of a novel type of cancer preventive medicine.
Rights: http://creativecommons.org/licenses/by/4.0/
Type: article
URI: http://hdl.handle.net/2115/60284
Appears in Collections:遺伝子病制御研究所 (Institute for Genetic Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 藤田 恭之

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