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Volume 63 Number 4 >

Comparing spatial expression dynamics of bovine blastocyst under three different procedures : in-vivo, in-vitro derived, and somatic cell nuclear transfer embryos

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Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.63.4.159

Title: Comparing spatial expression dynamics of bovine blastocyst under three different procedures : in-vivo, in-vitro derived, and somatic cell nuclear transfer embryos
Authors: Nagatomo, Hiroaki Browse this author
Akizawa, Hiroki Browse this author
Sada, Ayari Browse this author
Kishi, Yasunori Browse this author
Yamanaka, Ken-ichi Browse this author
Takuma, Tetsuya Browse this author
Sasaki, Keisuke Browse this author
Yamauchi, Nobuhiko Browse this author →KAKEN DB
Yanagawa, Yojiro Browse this author
Nagano, Masashi Browse this author →KAKEN DB
Kono, Tomohiro Browse this author →KAKEN DB
Takahashi, Masashi Browse this author →KAKEN DB
Kawahara, Manabu Browse this author →KAKEN DB
Keywords: cattle
blastocyst
cell lineage specification
gene expression
Issue Date: Nov-2015
Publisher: Graduate School of Veterinary Medicine, Hokkaido University
Journal Title: Japanese Journal of Veterinary Research
Volume: 63
Issue: 4
Start Page: 159
End Page: 171
Abstract: There has been no work on spatiotemporal transcriptomic differences of blastocysts using in vivo- and in vitro-derived, and somatic cell nuclear transfer (SCNT) embryos. Here, we first compared the lineage-differentially transcriptomic profiles of in vivo- and in vitroderived embryos by microarray analysis using divided into inner cell mass (ICM)-and trophectoderm (TE)-side samples, as well as those derived from SCNT in order to explore lineage-differentially expressed genes that are associated with preimplantation development in cattle. The transcriptomic profiles of the ICM-specific and TE-specific genes were similar between in vitro-derived embryos and in vivo-derived embryos, whereas SCNT embryos exhibited unusual lineage-differentially gene expression regulation at the blastocyst stage. The genes expressed in a spatiotemporal manner between developmentally normal in-vivo derived blastocysts and developmentally abnormal SCNT blastocysts might play critical roles for preimplantation development. Comparing spatial expression dynamics of bovine blastocyst under three different procedures revealed that CIITA was expressed in ICM-side samples of all the embryo types. CIITA is known as the master regulator of major histocompatibility complexes (MHC) class II genes that express in antigen-presenting cells but its biological function in preimplantation embryo is still unknown in mammals. Knockdown of CIITA expression in in vitro-derived embryos did not affect cleavage, but disrupted development of embryos into the blastocyst stage. These findings provide the novel transcriptomic information on blastocyst formation, raising the possibility that immune function-related gene directly plays important roles in bovine preimplantation development.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/60304
Appears in Collections:Japanese Journal of Veterinary Research > Volume 63 Number 4

Submitter: 獣医学部図書室

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