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Rapid immunohistochemistry based on alternating current electric field for intraoperative diagnosis of brain tumors

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タイトル: Rapid immunohistochemistry based on alternating current electric field for intraoperative diagnosis of brain tumors
著者: Tanino, Mishie 著作を一覧する
Sasajima, Toshio 著作を一覧する
Nanjo, Hiroshi 著作を一覧する
Akesaka, Shiori 著作を一覧する
Kagaya, Masami 著作を一覧する
Kimura, Taichi 著作を一覧する
Ishida, Yusuke 著作を一覧する
Oda, Masaya 著作を一覧する
Takahashi, Masataka 著作を一覧する
Sugawara, Taku 著作を一覧する
Yoshioka, Toshiaki 著作を一覧する
Nishihara, Hiroshi 著作を一覧する
Akagami, Yoichi 著作を一覧する
Goto, Akiteru 著作を一覧する
Minamiya, Yoshihiro 著作を一覧する
Tanaka, Shinya 著作を一覧する
キーワード: Rapid immunohistochemistry (R-IHC)
Central nervous system-lymphoma (CNS-lymphoma)
発行日: 2015年 1月
出版者: Springer Japan
誌名: Brain tumor pathology
巻: 32
号: 1
開始ページ: 12
終了ページ: 19
出版社 DOI: 10.1007/s10014-014-0188-y
抄録: Rapid immunohistochemistry (R-IHC) can contribute to the intraoperative diagnosis of central nervous system (CNS) tumors. We have recently developed a new IHC method based on an alternating current electric field to facilitate the antigen-antibody reaction. To ensure the requirement of R-IHC for intraoperative diagnosis, 183 cases of CNS tumors were reviewed regarding the accuracy rate of diagnosis without R-IHC. The diagnostic accuracy was 90.7 % (168/183 cases) in which definitive diagnoses were not provided in 17 cases because of the failure of glioma grading and differential diagnosis of lymphoma and glioma. To establish the clinicopathological application, R-IHC for frozen specimens was compared with standard IHC for permanent specimens. 33 gliomas were analyzed, and the Ki-67/MIB-1 indices of frozen specimens by R-IHC were consistent with the grade and statistically correlated with those of permanent specimens. Thus, R-IHC provided supportive information to determine the grade of glioma. For discrimination between glioma and lymphoma, R-IHC was able to provide clear results of CD20 and Ki-67/MIB-1 in four frozen specimens of CNS lymphoma as well as standard IHC. We conclude that the R-IHC for frozen specimens can provide important information for intraoperative diagnosis of CNS tumors.
Rights: The final publication is available at
資料タイプ: article (author version)
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 谷野 美智枝


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