| Title: | Rapid immunohistochemistry based on alternating current electric field for intraoperative diagnosis of brain tumors |
| Authors: | Tanino, Mishie Browse this author →KAKEN DB |
| Sasajima, Toshio Browse this author →KAKEN DB |
| Nanjo, Hiroshi Browse this author →KAKEN DB |
| Akesaka, Shiori Browse this author |
| Kagaya, Masami Browse this author |
| Kimura, Taichi Browse this author →KAKEN DB |
| Ishida, Yusuke Browse this author |
| Oda, Masaya Browse this author |
| Takahashi, Masataka Browse this author →KAKEN DB |
| Sugawara, Taku Browse this author →KAKEN DB |
| Yoshioka, Toshiaki Browse this author →KAKEN DB |
| Nishihara, Hiroshi Browse this author →KAKEN DB |
| Akagami, Yoichi Browse this author →KAKEN DB |
| Goto, Akiteru Browse this author →KAKEN DB |
| Minamiya, Yoshihiro Browse this author →KAKEN DB |
| Tanaka, Shinya Browse this author →KAKEN DB |
| Keywords: | Rapid immunohistochemistry (R-IHC) |
| Glioma |
| Central nervous system-lymphoma (CNS-lymphoma) |
| Issue Date: | Jan-2015 |
| Publisher: | Springer Japan |
| Journal Title: | Brain tumor pathology |
| Volume: | 32 |
| Issue: | 1 |
| Start Page: | 12 |
| End Page: | 19 |
| Publisher DOI: | 10.1007/s10014-014-0188-y |
| PMID: | 24807101 |
| Abstract: | Rapid immunohistochemistry (R-IHC) can contribute to the intraoperative diagnosis of central nervous system (CNS) tumors. We have recently developed a new IHC method based on an alternating current electric field to facilitate the antigen-antibody reaction. To ensure the requirement of R-IHC for intraoperative diagnosis, 183 cases of CNS tumors were reviewed regarding the accuracy rate of diagnosis without R-IHC. The diagnostic accuracy was 90.7 % (168/183 cases) in which definitive diagnoses were not provided in 17 cases because of the failure of glioma grading and differential diagnosis of lymphoma and glioma. To establish the clinicopathological application, R-IHC for frozen specimens was compared with standard IHC for permanent specimens. 33 gliomas were analyzed, and the Ki-67/MIB-1 indices of frozen specimens by R-IHC were consistent with the grade and statistically correlated with those of permanent specimens. Thus, R-IHC provided supportive information to determine the grade of glioma. For discrimination between glioma and lymphoma, R-IHC was able to provide clear results of CD20 and Ki-67/MIB-1 in four frozen specimens of CNS lymphoma as well as standard IHC. We conclude that the R-IHC for frozen specimens can provide important information for intraoperative diagnosis of CNS tumors. |
| Rights: | The final publication is available at link.springer.com |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/60447 |
| Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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