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Rapid immunohistochemistry based on alternating current electric field for intraoperative diagnosis of brain tumors

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/60447

Title: Rapid immunohistochemistry based on alternating current electric field for intraoperative diagnosis of brain tumors
Authors: Tanino, Mishie Browse this author →KAKEN DB
Sasajima, Toshio Browse this author →KAKEN DB
Nanjo, Hiroshi Browse this author →KAKEN DB
Akesaka, Shiori Browse this author
Kagaya, Masami Browse this author
Kimura, Taichi Browse this author →KAKEN DB
Ishida, Yusuke Browse this author
Oda, Masaya Browse this author
Takahashi, Masataka Browse this author →KAKEN DB
Sugawara, Taku Browse this author →KAKEN DB
Yoshioka, Toshiaki Browse this author →KAKEN DB
Nishihara, Hiroshi Browse this author →KAKEN DB
Akagami, Yoichi Browse this author →KAKEN DB
Goto, Akiteru Browse this author →KAKEN DB
Minamiya, Yoshihiro Browse this author →KAKEN DB
Tanaka, Shinya Browse this author →KAKEN DB
Keywords: Rapid immunohistochemistry (R-IHC)
Glioma
Central nervous system-lymphoma (CNS-lymphoma)
Issue Date: Jan-2015
Publisher: Springer Japan
Journal Title: Brain tumor pathology
Volume: 32
Issue: 1
Start Page: 12
End Page: 19
Publisher DOI: 10.1007/s10014-014-0188-y
PMID: 24807101
Abstract: Rapid immunohistochemistry (R-IHC) can contribute to the intraoperative diagnosis of central nervous system (CNS) tumors. We have recently developed a new IHC method based on an alternating current electric field to facilitate the antigen-antibody reaction. To ensure the requirement of R-IHC for intraoperative diagnosis, 183 cases of CNS tumors were reviewed regarding the accuracy rate of diagnosis without R-IHC. The diagnostic accuracy was 90.7 % (168/183 cases) in which definitive diagnoses were not provided in 17 cases because of the failure of glioma grading and differential diagnosis of lymphoma and glioma. To establish the clinicopathological application, R-IHC for frozen specimens was compared with standard IHC for permanent specimens. 33 gliomas were analyzed, and the Ki-67/MIB-1 indices of frozen specimens by R-IHC were consistent with the grade and statistically correlated with those of permanent specimens. Thus, R-IHC provided supportive information to determine the grade of glioma. For discrimination between glioma and lymphoma, R-IHC was able to provide clear results of CD20 and Ki-67/MIB-1 in four frozen specimens of CNS lymphoma as well as standard IHC. We conclude that the R-IHC for frozen specimens can provide important information for intraoperative diagnosis of CNS tumors.
Rights: The final publication is available at link.springer.com
Type: article (author version)
URI: http://hdl.handle.net/2115/60447
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 谷野 美智枝

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