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Three-dimensional morphogenesis of MDCK cells induced by cellular contractile forces on a viscous substrate
This item is licensed under:Creative Commons Attribution 4.0 International
| Title: | Three-dimensional morphogenesis of MDCK cells induced by cellular contractile forces on a viscous substrate |
| Authors: | Imai, Misako Browse this author | | Furusawa, Kazuya Browse this author | | Mizutani, Takeomi Browse this author | | Kawabata, Kazushige Browse this author | | Haga, Hisashi Browse this author →KAKEN DB |
| Issue Date: | 16-Sep-2015 |
| Publisher: | Nature Publishing Group |
| Journal Title: | Scientific reports |
| Volume: | 5 |
| Start Page: | 14208 |
| Publisher DOI: | 10.1038/srep14208 |
| Abstract: | Substrate physical properties are essential for many physiological events such as embryonic development and 3D tissue formation. Physical properties of the extracellular matrix such as viscoelasticity and geometrical constraints are understood as factors that affect cell behaviour. In this study, we focused on the relationship between epithelial cell 3D morphogenesis and the substrate viscosity. We observed that Madin-Darby Canine Kidney (MDCK) cells formed 3D structures on a viscous substrate (Matrigel). The structures appear as a tulip hat. We then changed the substrate viscosity by genipin (GP) treatment. GP is a cross-linker of amino groups. Cells cultured on GP-treated-matrigel changed their 3D morphology in a substrate viscosity-dependent manner. Furthermore, to elucidate the spatial distribution of the cellular contractile force, localization of mono-phosphorylated and di-phosphorylated myosin regulatory light chain (P-MRLCs) was visualized by immunofluorescence. P-MRLCs localized along the periphery of epithelial sheets. Treatment with Y-27632, a Rho-kinase inhibitor, blocked the P-MRLCs localization at the edge of epithelial sheets and halted 3D morphogenesis. Our results indicate that the substrate viscosity, the substrate deformation, and the cellular contractile forces induced by P-MRLCs play crucial roles in 3D morphogenesis. |
| Rights: | http://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/60455 |
| Appears in Collections: | 生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 芳賀 永
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