HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Life Science / Faculty of Advanced Life Science >
Peer-reviewed Journal Articles, etc >

Raster image cross-correlation analysis for spatiotemporal visualization of intracellular degradation activities against exogenous DNAs

This item is licensed under: Creative Commons Attribution 4.0 International

Files in This Item:
srep14428.pdf1.29 MBPDFView/Open
Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/60461

Title: Raster image cross-correlation analysis for spatiotemporal visualization of intracellular degradation activities against exogenous DNAs
Authors: Sasaki, Akira Browse this author
Yamamoto, Johtaro Browse this author
Jin, Takashi Browse this author
Kinjo, Masataka Browse this author →KAKEN DB
Issue Date: 24-Sep-2015
Publisher: Nature Publishing Group
Journal Title: Scientific reports
Volume: 5
Start Page: 14428
Publisher DOI: 10.1038/srep14428
PMID: 26400011
Abstract: Reducing intracellular DNA degradation is critical to enhance the efficiency of gene therapy. Exogenous DNA incorporation into cells is strictly blocked by the defense machinery of intracellular nuclease activity. Raster image correlation spectroscopy (RICS) and raster image cross-correlation spectroscopy (cross-correlation RICS; ccRICS) are image-based correlation methods. These powerful tools allow the study of spatiotemporal molecular dynamics. Here we performed spatiotemporal ccRICS analyses of fluorescent DNA and directly monitored the process of exogenous DNA degradation in living cell cytoplasm. Such direct monitors of DNA degradation allow us to determine the fate of the exogenous DNA in living cells. On comparing the process in living cells, our study shows that cytoplasmic nuclease activity differs between cell lines; therefore, we propose that the difference of nuclease activity in cytoplasm dictates a different resistance to exogenous DNA incorporation. New insight on efficient gene delivery can be provided with our study.
Rights: http://creativecommons.org/licenses/by/4.0/
Type: article
URI: http://hdl.handle.net/2115/60461
Appears in Collections:生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 金城 政孝

Export metadata:

OAI-PMH ( junii2 , jpcoar )

MathJax is now OFF:


 

 - Hokkaido University