HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Dental Medicine / Faculty of Dental Medicine >
Peer-reviewed Journal Articles, etc >

Optimal compressive force accelerates osteoclastogenesis in RAW264.7 cells

Files in This Item:
Mol Med Report Vol12 No4 Pg5879-2.pdf653.05 kBPDFView/Open
Please use this identifier to cite or link to this item:

Title: Optimal compressive force accelerates osteoclastogenesis in RAW264.7 cells
Authors: Hayakawa, Takako Browse this author
Yoshimura, Yoshitaka Browse this author →KAKEN DB
Kikuiri, Takashi Browse this author →KAKEN DB
Matsuno, Mino Browse this author →KAKEN DB
Hasegawa, Tomokazu Browse this author
Fukushima, Kumu Browse this author
Shibata, Kenjiro Browse this author
Deyama, Yoshiaki Browse this author →KAKEN DB
Suzuki, Kuniaki Browse this author →KAKEN DB
Iida, Junichiro Browse this author →KAKEN DB
Keywords: optimal compressive force
collagen gel
Issue Date: Oct-2015
Publisher: Spandidos Publications
Journal Title: Molecular medicine reports
Volume: 12
Issue: 4
Start Page: 5879
End Page: 5885
Publisher DOI: 10.3892/mmr.2015.4141
PMID: 26238100
Abstract: Mechanical stress produced by orthodontic forces is a factor in the remodeling of periodontal ligaments (PDLs) and alveolar bone. It has been reported that the expression of a number of cytokines associated with osteoclastogenesis is upregulated when compressive forces act on osteoblasts and PDL cells. The present study investigated the effects of compressive forces on the formation of osteoclasts from the macrophage cell line RAW264.7. Compressive forces on osteoclasts were exerted using layers of 3, 5, 7, 9 or 14 glass cover slips on the 4th day of culture for 24 h. The number of osteoclasts was determined by counting the number of cells positive for tartrate-resistant acid phosphatase staining. Osteoclastogenesis advanced rapidly on days four and five. The number of osteoclasts with >8 nuclei peaked when the force of 7 slips was applied, which was therefore regarded as the optimal compressive force. Alterations in the expression of osteoclast-associated genes are associated with changes in the differentiation and fusion of macrophages in response to compressive forces; therefore, osteoclast-associated genes were assessed by reverse transcription quantitative polymerase chain reaction in the present study. The mRNA expression of osteoclast-associated genes increased significantly after 3 h of optimal compression, whereas mRNA expression increased after 24 h in the control group. These findings suggested that osteoclastogenesis of macrophages was accelerated when an optimal compressive force was applied.
Type: article
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 吉村 善隆

Export metadata:


Feedback - Hokkaido University