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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Dental Medicine / Faculty of Dental Medicine >
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Optimal compressive force accelerates osteoclastogenesis in RAW264.7 cells
| Title: | Optimal compressive force accelerates osteoclastogenesis in RAW264.7 cells |
| Authors: | Hayakawa, Takako Browse this author | | Yoshimura, Yoshitaka Browse this author →KAKEN DB | | Kikuiri, Takashi Browse this author →KAKEN DB | | Matsuno, Mino Browse this author →KAKEN DB | | Hasegawa, Tomokazu Browse this author | | Fukushima, Kumu Browse this author | | Shibata, Kenjiro Browse this author | | Deyama, Yoshiaki Browse this author →KAKEN DB | | Suzuki, Kuniaki Browse this author →KAKEN DB | | Iida, Junichiro Browse this author →KAKEN DB |
| Keywords: | optimal compressive force | | osteoclastogenesis | | collagen gel |
| Issue Date: | Oct-2015 |
| Publisher: | Spandidos Publications |
| Journal Title: | Molecular medicine reports |
| Volume: | 12 |
| Issue: | 4 |
| Start Page: | 5879 |
| End Page: | 5885 |
| Publisher DOI: | 10.3892/mmr.2015.4141 |
| PMID: | 26238100 |
| Abstract: | Mechanical stress produced by orthodontic forces is a factor in the remodeling of periodontal ligaments (PDLs) and alveolar bone. It has been reported that the expression of a number of cytokines associated with osteoclastogenesis is upregulated when compressive forces act on osteoblasts and PDL cells. The present study investigated the effects of compressive forces on the formation of osteoclasts from the macrophage cell line RAW264.7. Compressive forces on osteoclasts were exerted using layers of 3, 5, 7, 9 or 14 glass cover slips on the 4th day of culture for 24 h. The number of osteoclasts was determined by counting the number of cells positive for tartrate-resistant acid phosphatase staining. Osteoclastogenesis advanced rapidly on days four and five. The number of osteoclasts with >8 nuclei peaked when the force of 7 slips was applied, which was therefore regarded as the optimal compressive force. Alterations in the expression of osteoclast-associated genes are associated with changes in the differentiation and fusion of macrophages in response to compressive forces; therefore, osteoclast-associated genes were assessed by reverse transcription quantitative polymerase chain reaction in the present study. The mRNA expression of osteoclast-associated genes increased significantly after 3 h of optimal compression, whereas mRNA expression increased after 24 h in the control group. These findings suggested that osteoclastogenesis of macrophages was accelerated when an optimal compressive force was applied. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/60591 |
| Appears in Collections: | 歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 吉村 善隆
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