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Structural Characterization Reveals the Keratinolytic Activity of an Arthrobacter nicotinovorans Protease

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Title: Structural Characterization Reveals the Keratinolytic Activity of an Arthrobacter nicotinovorans Protease
Authors: Sone, Teruo Browse this author →KAKEN DB
Haraguchi, Yumiko Browse this author
Kuwahara, Aki Browse this author
Ose, Toyoyuki Browse this author →KAKEN DB
Takano, Megumi Browse this author
Abe, Ayumi Browse this author
Tanaka, Michiko Browse this author
Tanaka, Isao Browse this author →KAKEN DB
Asano, Kozo Browse this author →KAKEN DB
Keywords: Arthrobacter nicotinovorans
cadmium
crystallization
metallothionein
scallops
serine protease
Issue Date: Jan-2015
Publisher: Bentham Science Publishers
Journal Title: Protein and peptide letters
Volume: 22
Issue: 1
Start Page: 63
End Page: 72
Publisher DOI: 10.2174/0929866521666140919100851
Abstract: Elevated cadmium (Cd) concentrations in fishery byproducts are an environmental concern, that might be reduced by enzymatic removal and adsorption of the contaminants during recycling the byproducts as animal food. We cloned the gene for Arthrobacter nicotinovorans serine protease (ANISEP), which was isolated from the hepatopancreas of the Japanese scallop (Patiopecten yessoensis) and has been found to be an effective enzyme for Cd(II) removal. The gene is 993 bp in length and encodes 330 amino acids, including the pre (1-30) and pro (31-111) sequences. The catalytic triad consists of His, Asp, and Ser. Sequence similarities indicate that ANISEP is a extracellular serine protease. X-ray crystallography revealed structural similarities between ANISEP and the trypsin-like serine protease NAALP from Nesterenkonia sp. Site-directed mutagenesis identified Ser171 as catalytic residue. The keratinolytic activity of ANISEP was 10-fold greater than that of trypsin. ANISEP digested Cd(II)-bound recombinant metallothionein MT-10a from Laternula elliptica, but did not release Cd. These results further suggest ANISEP is a trypsin-like serine protease that can release Cd from the Japanese scallop hepatopancreas because of its strong keratinolytic activity.
Rights: The published manuscript is available at EurekaSelect via http://www.eurekaselect.com/openurl/content.php?genre=article&doi=10.2174/0929866521666140919100851.
Relation: http://benthamscience.com/index.php
Type: article (author version)
URI: http://hdl.handle.net/2115/60612
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 曾根 輝雄

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