| Title: | CTNNB1 mutational analysis of solid-pseudopapillary neoplasms of the pancreas using endoscopic ultrasound-guided fine-needle aspiration and next-generation deep sequencing |
| Authors: | Kubota, Yoshimasa Browse this author |
| Kawakami, Hiroshi Browse this author →KAKEN DB |
| Natsuizaka, Mitsuteru Browse this author |
| Kawakubo, Kazumichi Browse this author |
| Marukawa, Katsuji Browse this author |
| Kudo, Taiki Browse this author |
| Abe, Yoko Browse this author |
| Kubo, Kimitoshi Browse this author |
| Kuwatani, Masaki Browse this author →KAKEN DB |
| Hatanaka, Yutaka Browse this author →KAKEN DB |
| Mitsuhashi, Tomoko Browse this author →KAKEN DB |
| Matsuno, Yoshihiro Browse this author |
| Sakamoto, Naoya Browse this author →KAKEN DB |
| Keywords: | CTNNB1 |
| Solid-pseudopapillary neoplasms of the pancreas |
| Endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA) |
| Next-generation sequencing |
| Issue Date: | Feb-2015 |
| Publisher: | Springer |
| Journal Title: | Journal of Gastroenterology |
| Volume: | 50 |
| Issue: | 2 |
| Start Page: | 203 |
| End Page: | 210 |
| Publisher DOI: | 10.1007/s00535-014-0954-y |
| PMID: | 24700283 |
| Abstract: | Solid-pseudopapillary neoplasm (SPN), a rare neoplasm of the pancreas, frequently harbors mutations in exon 3 of the cadherin-associated protein beta 1 (CTNNB1) gene. Here, we analyzed SPN tissue for CTNNB1 mutations by deep sequencing using next-generation sequencing (NGS). Tissue samples from 7 SPNs and 31 other pancreatic lesions (16 pancreatic ductal adenocarcinomas (PDAC), 11 pancreatic neuroendocrine tumors (PNET), 1 acinar cell carcinoma, 1 autoimmune pancreatitis lesion, and 2 focal pancreatitis lesions) were analyzed by NGS for mutations in exon 3 of CTNNB1. A single-base-pair missense mutations in exon 3 of CTNNB1 was observed in all 7 SPNs and in 1 of 11 PNET samples. However, mutations were not observed in the tissue samples of any of the 16 PDAC or other four pancreatic disease cases. The variant frequency of CTNNB1 ranged from 5.4 to 48.8 %. Mutational analysis of CTNNB1 by NGS is feasible and was achieved using SPN samples obtained by endoscopic ultrasound-guided fine needle aspiration. |
| Rights: | The final publication is available at link.springer.com |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/60616 |
| Appears in Collections: | 北海道大学病院 (Hokkaido University Hospital) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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