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CTNNB1 mutational analysis of solid-pseudopapillary neoplasms of the pancreas using endoscopic ultrasound-guided fine-needle aspiration and next-generation deep sequencing

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/60616

Title: CTNNB1 mutational analysis of solid-pseudopapillary neoplasms of the pancreas using endoscopic ultrasound-guided fine-needle aspiration and next-generation deep sequencing
Authors: Kubota, Yoshimasa Browse this author
Kawakami, Hiroshi Browse this author →KAKEN DB
Natsuizaka, Mitsuteru Browse this author
Kawakubo, Kazumichi Browse this author
Marukawa, Katsuji Browse this author
Kudo, Taiki Browse this author
Abe, Yoko Browse this author
Kubo, Kimitoshi Browse this author
Kuwatani, Masaki Browse this author →KAKEN DB
Hatanaka, Yutaka Browse this author →KAKEN DB
Mitsuhashi, Tomoko Browse this author →KAKEN DB
Matsuno, Yoshihiro Browse this author
Sakamoto, Naoya Browse this author →KAKEN DB
Keywords: CTNNB1
Solid-pseudopapillary neoplasms of the pancreas
Endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA)
Next-generation sequencing
Issue Date: Feb-2015
Publisher: Springer
Journal Title: Journal of Gastroenterology
Volume: 50
Issue: 2
Start Page: 203
End Page: 210
Publisher DOI: 10.1007/s00535-014-0954-y
PMID: 24700283
Abstract: Solid-pseudopapillary neoplasm (SPN), a rare neoplasm of the pancreas, frequently harbors mutations in exon 3 of the cadherin-associated protein beta 1 (CTNNB1) gene. Here, we analyzed SPN tissue for CTNNB1 mutations by deep sequencing using next-generation sequencing (NGS). Tissue samples from 7 SPNs and 31 other pancreatic lesions (16 pancreatic ductal adenocarcinomas (PDAC), 11 pancreatic neuroendocrine tumors (PNET), 1 acinar cell carcinoma, 1 autoimmune pancreatitis lesion, and 2 focal pancreatitis lesions) were analyzed by NGS for mutations in exon 3 of CTNNB1. A single-base-pair missense mutations in exon 3 of CTNNB1 was observed in all 7 SPNs and in 1 of 11 PNET samples. However, mutations were not observed in the tissue samples of any of the 16 PDAC or other four pancreatic disease cases. The variant frequency of CTNNB1 ranged from 5.4 to 48.8 %. Mutational analysis of CTNNB1 by NGS is feasible and was achieved using SPN samples obtained by endoscopic ultrasound-guided fine needle aspiration.
Rights: The final publication is available at link.springer.com
Type: article (author version)
URI: http://hdl.handle.net/2115/60616
Appears in Collections:北海道大学病院 (Hokkaido University Hospital) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 河上 洋

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