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Detection and Characterization of Mycoviruses in Arbuscular Mycorrhizal Fungi by Deep-Sequencing

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/60635

Title: Detection and Characterization of Mycoviruses in Arbuscular Mycorrhizal Fungi by Deep-Sequencing
Authors: Ezawa, Tatsuhiro Browse this author →KAKEN DB
Ikeda, Yoji Browse this author
Shimura, Hanako Browse this author
Masuta, Chikara Browse this author →KAKEN DB
Keywords: Arbuscular mycorrhizal fungi
Deep-sequencing
dsRNA
Mycoviruses
Obligate biotroph
Issue Date: 2015
Publisher: springer
Journal Title: Methods in Molecular Biology
Volume: 1236
Start Page: 171
End Page: 180
Publisher DOI: 10.1007/978-1-4939-1743-3_13
PMID: 25287503
Abstract: Fungal viruses (mycoviruses) often have a significant impact not only on phenotypic expression of the host fungus but also on higher order biological interactions, e.g., conferring plant stress tolerance via an endophytic host fungus. Arbuscular mycorrhizal (AM) fungi in the phylum Glomeromycota associate with most land plants and supply mineral nutrients to the host plants. So far, little information about mycoviruses has been obtained in the fungi due to their obligate biotrophic nature. Here we provide a technical breakthrough, “two-step strategy” in combination with deep-sequencing, for virological study in AM fungi; dsRNA is first extracted and sequenced using material obtained from highly productive open pot culture, and then the presence of viruses is verified using pure material produced in the in vitro monoxenic culture. This approach enabled us to demonstrate the presence of several viruses for the first time from a glomeromycotan fungus.
Rights: The final publication is available at www.springerlink.com
Type: article (author version)
URI: http://hdl.handle.net/2115/60635
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 江澤 辰広

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