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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
Peer-reviewed Journal Articles, etc >
Detection and Characterization of Mycoviruses in Arbuscular Mycorrhizal Fungi by Deep-Sequencing
| Title: | Detection and Characterization of Mycoviruses in Arbuscular Mycorrhizal Fungi by Deep-Sequencing |
| Authors: | Ezawa, Tatsuhiro Browse this author →KAKEN DB | | Ikeda, Yoji Browse this author | | Shimura, Hanako Browse this author | | Masuta, Chikara Browse this author →KAKEN DB |
| Keywords: | Arbuscular mycorrhizal fungi | | Deep-sequencing | | dsRNA | | Mycoviruses | | Obligate biotroph |
| Issue Date: | 2015 |
| Publisher: | springer |
| Journal Title: | Methods in Molecular Biology |
| Volume: | 1236 |
| Start Page: | 171 |
| End Page: | 180 |
| Publisher DOI: | 10.1007/978-1-4939-1743-3_13 |
| PMID: | 25287503 |
| Abstract: | Fungal viruses (mycoviruses) often have a significant impact not only on phenotypic expression of the host fungus but also on higher order biological interactions, e.g., conferring plant stress tolerance via an endophytic host fungus. Arbuscular mycorrhizal (AM) fungi in the phylum Glomeromycota associate with most land plants and supply mineral nutrients to the host plants. So far, little information about mycoviruses has been obtained in the fungi due to their obligate biotrophic nature. Here we provide a technical breakthrough, “two-step strategy” in combination with deep-sequencing, for virological study in AM fungi; dsRNA is first extracted and sequenced using material obtained from highly productive open pot culture, and then the presence of viruses is verified using pure material produced in the in vitro monoxenic culture. This approach enabled us to demonstrate the presence of several viruses for the first time from a glomeromycotan fungus. |
| Rights: | The final publication is available at www.springerlink.com |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/60635 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 江澤 辰広
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