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Rice ragged stunt Oryzavirus genome segment 9 encodes a 38 600 Mr structural protein

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Title: Rice ragged stunt Oryzavirus genome segment 9 encodes a 38 600 Mr structural protein
Authors: Uyeda, I.1 Browse this author →KAKEN DB
Suga, H. Browse this author
Lee, SY Browse this author
Yan, J. Browse this author
Hataya, T. Browse this author
Kimura, I. Browse this author
Shikata, E. Browse this author
Authors(alt): 上田, 一郎1
Issue Date: May-1995
Publisher: Society for General Microbiology
Journal Title: Journal of General Virology
Volume: 76
Start Page: 975
End Page: 978
Abstract: The complete nucleotide sequence of rice ragged stunt virus genome segment 9(S9) was determined. The S9 segment is 1132 nucleotides long and has a long open reading frame starting from the first AUG codon at nucleotide position 14-16 and terminating at a UAG codon located at 1028-1030, which could encode a polypeptide with an Mr of 38 600 (P9). The encoded polypeptide has no sequence homology to polypeptides of any other plant reoviruses published previously. An immunological study demonstrated that P9 was the smallest of the structural proteins. The P9 polypeptide was expressed as a fusion protein with maltose binding protein in Escherichia coli. Antisera to purified virions and to the fusion protein reacted with both the bacterially expressed polypeptide and the smallest polypeptide of the purified virus in immunoblotting analyses.
Type: article
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 上田 一郎

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