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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
Peer-reviewed Journal Articles, etc >
Rice ragged stunt Oryzavirus genome segment 9 encodes a 38 600 Mr structural protein
| Title: | Rice ragged stunt Oryzavirus genome segment 9 encodes a 38 600 Mr structural protein |
| Authors: | Uyeda, I.1 Browse this author →KAKEN DB | | Suga, H. Browse this author | | Lee, SY Browse this author | | Yan, J. Browse this author | | Hataya, T. Browse this author | | Kimura, I. Browse this author | | Shikata, E. Browse this author |
| Authors(alt): | 上田, 一郎1 |
| Issue Date: | May-1995 |
| Publisher: | Society for General Microbiology |
| Journal Title: | Journal of General Virology |
| Volume: | 76 |
| Start Page: | 975 |
| End Page: | 978 |
| Abstract: | The complete nucleotide sequence of rice ragged stunt virus genome segment 9(S9) was determined. The S9 segment is 1132 nucleotides long and has a long open reading frame starting from the first AUG codon at nucleotide position 14-16 and terminating at a UAG codon located at 1028-1030, which could encode a polypeptide with an Mr of 38 600 (P9). The encoded polypeptide has no sequence homology to polypeptides of any other plant reoviruses published previously. An immunological study demonstrated that P9 was the smallest of the structural proteins. The P9 polypeptide was expressed as a fusion protein with maltose binding protein in Escherichia coli. Antisera to purified virions and to the fusion protein reacted with both the bacterially expressed polypeptide and the smallest polypeptide of the purified virus in immunoblotting analyses. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/6064 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 上田 一郎
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