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Monocyte/macrophage-Specific NADPH Oxidase Contributes to Antimicrobial Host Defense in X-CGD

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/60655

Title: Monocyte/macrophage-Specific NADPH Oxidase Contributes to Antimicrobial Host Defense in X-CGD
Authors: Okura, Yuka Browse this author
Yamada, Masafumi Browse this author
Kuribayashi, Futoshi Browse this author →KAKEN DB
Kobayashi, Ichiro Browse this author
Ariga, Tadashi Browse this author →KAKEN DB
Keywords: X-linked chronic granulomatous disease
monocyte
macrophage
NADPH oxidase
Issue Date: Feb-2015
Publisher: Springer US
Journal Title: Journal of clinical immunology
Volume: 35
Issue: 2
Start Page: 158
End Page: 167
Publisher DOI: 10.1007/s10875-015-0138-4
PMID: 25666294
Abstract: Chronic granulomatous disease (CGD) is a primary immunodeficiency disease that is characterized by susceptibility to bacterial and fungal infections. Various mutations in CYBB encoding the gp91(phox) subunit of the phagocyte nicotinamide adenine dinucleotide phosphate (NADPH) oxidase impair the respiratory burst of all types of phagocytic cells and result in X-linked CGD (X-CGD). We here sought to evaluate the underlying cause in an attenuated phenotype in an X-CGD patient. The patient is a 31-year-old male who had been diagnosed as having X-CGD based on the absence of nitroblue tetrazolium reduction and the presence of a CYBB mutation at the age of 1 year. He has been in good health after overcoming recurrent bacterial infections in infancy. We investigated genomic DNA analysis of CYBB gene, residual activity of NADPH oxidase, and expression of gp91(phox) in both polymorphonuclear leukocytes (PMNs) and monocytes/macrophages in the present patient. Although his underlying germline mutation, c.1016C > A (p.P339H) in the CYBB gene, was identified in both PMNs and monocytes, the expression and functional activity of gp91(phox) retained in monocytes/macrophages, in stark contrast to markedly reduced PMNs. Our results indicate that residual reactive oxygen intermediates (ROI) production in PMNs plays an important role in infantile stage in X-CGD, but thereafter retained function of monocytes/macrophages might compensate for the function of NADPH oxidase deficient PMNs and might be an important parameter for predicting the prognosis of X-CGD patients.
Rights: The final publication is available at Springer via http://dx.doi.org/10.1007/s10875-015-0138-4
Type: article (author version)
URI: http://hdl.handle.net/2115/60655
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 大倉 有加

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