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ATP hydrolysis assists phosphate release and promotes reaction ordering in F-1-ATPase

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Title: ATP hydrolysis assists phosphate release and promotes reaction ordering in F-1-ATPase
Authors: Li, Chun-Biu Browse this author
Ueno, Hiroshi Browse this author
Watanabe, Rikiya Browse this author →KAKEN DB
Noji, Hiroyuki Browse this author →KAKEN DB
Komatsuzaki, Tamiki Browse this author →KAKEN DB
Issue Date: 17-Dec-2015
Publisher: Nature Publishing Group
Journal Title: Nature communications
Volume: 6
Start Page: 10223
Publisher DOI: 10.1038/ncomms10223
Abstract: F-1-ATPase (F-1) is a rotary motor protein that can efficiently convert chemical energy to mechanical work of rotation via fine coordination of its conformational motions and reaction sequences. Compared with reactant binding and product release, the ATP hydrolysis has relatively little contributions to the torque and chemical energy generation. To scrutinize possible roles of ATP hydrolysis, we investigate the detailed statistics of the catalytic dwells from high-speed single wild-type F-1 observations. Here we report a small rotation during the catalytic dwell triggered by the ATP hydrolysis that is indiscernible in previous studies. Moreover, we find in freely rotating F-1 that ATP hydrolysis is followed by the release of inorganic phosphate with low synthesis rates. Finally, we propose functional roles of the ATP hydrolysis as a key to kinetically unlock the subsequent phosphate release and promote the correct reaction ordering.
Type: article
Appears in Collections:電子科学研究所 (Research Institute for Electronic Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 小松崎 民樹

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