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Cellular and subcellular localization of cholecystokinin (CCK)-1 receptors in the pancreas, gallbladder, and stomach of mice

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/60738

Title: Cellular and subcellular localization of cholecystokinin (CCK)-1 receptors in the pancreas, gallbladder, and stomach of mice
Authors: Konno, Kohtarou Browse this author
Takahashi-Iwanaga, Hiromi Browse this author
Uchigashima, Motokazu Browse this author →KAKEN DB
Miyasaka, Kyoko Browse this author →KAKEN DB
Funakoshi, Akihiro Browse this author
Watanabe, Masahiko Browse this author →KAKEN DB
Iwanaga, Toshihiko Browse this author →KAKEN DB
Keywords: Cholecystokinin
CCK1 receptor
Pancreas
Stomach
Gallbladder
Issue Date: Mar-2015
Publisher: Springer
Journal Title: Histochemistry and Cell Biology
Volume: 143
Issue: 3
Start Page: 301
End Page: 312
Publisher DOI: 10.1007/s00418-014-1281-3
PMID: 25249350
Abstract: Information concerning the cellular localization of cholecystokinin (CCK)-1 receptors has been discrepant and remained scanty at ultrastructural levels. The present immunohistochemical study at light and electron microscopic levels revealed the distinct localization of CCK1 receptors in visceral organs. Immunohistochemistry by use of a purified antibody against mouse CCK1 receptor was applied to fixed tissue sections of the pancreas, gallbladder, stomach, and intestine of mice. A silver-intensified immunogold method revealed the subcellular localization under electron microscope. The immunoreactivity for CCK1 receptors was selectively found in the basolateral membrane of pancreatic acinar cells and gastric chief cells but was absent in pancreatic islets and gastric D cells. Another intense expression in the gut was seen in the myenteric nerve plexus of the antro-duodenal region and some populations of c-Kit-expressing pacemaker cells in the duodenal musculature. The gallbladder contained smooth muscle fibers with an intense immunoreactivity of CCK1 receptors on cell surfaces. The restricted localization of CCK1 receptors on the basolateral membrane of pancreatic acinar cells and gastric chief cells, along with their absence in the islets of Langerhans and gastric D cells, provides definitive information concerning the regulatory mechanism by circulating CCK. Especially, the subcellular localization in the acinar cells completes the investigation for the detection of circulating CCK by the basolateral membrane.
Rights: The final publication is available at link.springer.com
Type: article (author version)
URI: http://hdl.handle.net/2115/60738
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 岩永 敏彦

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