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Structural elements responsible for the glucosidic linkage-selectivity of a glycoside hydrolase family 13 exo-glucosidase
| Title: | Structural elements responsible for the glucosidic linkage-selectivity of a glycoside hydrolase family 13 exo-glucosidase |
| Authors: | Saburi, Wataru Browse this author →KAKEN DB | | Rachi-Otsuka, Hiroaki Browse this author | | Hondoh, Hironori Browse this author | | Okuyama, Masayuki Browse this author →KAKEN DB | | Mori, Haruhide Browse this author →KAKEN DB | | Kimura, Atsuo Browse this author →KAKEN DB |
| Keywords: | Dextran glucosidase | | alpha-Glucosidase | | Oligo-1,6-glucosidase | | Substrate specificity | | Glycoside hydrolase family 13 |
| Issue Date: | 25-Mar-2015 |
| Publisher: | Elsevier |
| Journal Title: | Febs letters |
| Volume: | 589 |
| Issue: | 7 |
| Start Page: | 865 |
| End Page: | 869 |
| Publisher DOI: | 10.1016/j.febslet.2015.02.023 |
| PMID: | 25728274 |
| Abstract: | Glycoside hydrolase family 13 contains exo-glucosidases specific for alpha-(1 -> 4)- and alpha-(1 -> 6)-linkages including alpha-glucosidase, oligo-1,6-glucosidase, and dextran glucosidase. The alpha-(1 -> 6)-linkage selectivity of Streptococcus mutans dextran glucosidase was altered to alpha-(1 -> 4)-linkage selectivity through site-directed mutations at Val195, Lys275, and Glu371. V195A showed 1300-fold higher k(cat)/K-m for maltose than wild-type, but its k(cat)/K-m for isomaltose remained 2-fold higher than for maltose. K275A and E371A combined with V195A mutation only decreased isomaltase activity. V195A/K275A, V195A/E371A, and V195A/K275A/E371A showed 27-, 26-, and 73-fold higher k(cat)/K-m for maltose than for isomaltose, respectively. Consequently, the three residues are structural elements for recognition of the alpha-(1 -> 6)-glucosidic linkage. (C) 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/60921 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 木村 淳夫
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