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Characterization of a Cyanobacterial Chloride-pumping Rhodopsin and Its Conversion into a Proton Pump
Title: | Characterization of a Cyanobacterial Chloride-pumping Rhodopsin and Its Conversion into a Proton Pump |
Authors: | Hasemi, Takatoshi Browse this author | Kikukawa, Takashi Browse this author →KAKEN DB | Kamo, Naoki Browse this author | Demura, Makoto Browse this author |
Keywords: | bioenergetics | membrane protein | photobiology | photoreceptor | proton pump | microbial rhodopsin | retinal proteins |
Issue Date: | 1-Jan-2016 |
Publisher: | American Society for Biochemistry and Molecular Biology (ASBMB) |
Journal Title: | Journal of biological chemistry |
Volume: | 291 |
Issue: | 1 |
Start Page: | 355 |
End Page: | 362 |
Publisher DOI: | 10.1074/jbc.M115.688614 |
PMID: | 26578511 |
Abstract: | Light-driven ion-pumping rhodopsins are widely distributed in microorganisms and are now classified into the categories of outward H+ and Na+ pumps and an inward Cl- pump. These different types share a common protein architecture and utilize the photoisomerization of the same chromophore, retinal, to evoke photoreactions. Despite these similarities, successful pump-to-pump conversion had been confined to only the H+ pump bacteriorhodopsin, which was converted to a Cl- pump in 1995 by a single amino acid replacement. In this study we report the first success of the reverse conversion from a Cl- pump to a H+ pump. A novel microbial rhodopsin (MrHR) from the cyanobacterium Mastigocladopsis repens functions as a Cl- pump and belongs to a cluster that is far distant from the known Cl- pumps. With a single amino acid replacement, MrHR is converted to a H+ pump in which dissociable residues function almost completely in the H+ relay reactions. MrHR most likely evolved from a H+ pump, but it has not yet been highly optimized into a mature Cl- pump. |
Rights: | This research was originally published in Journal of Biological Chemistry. Takatoshi Hasemi, Takashi Kikukawa1, Naoki Kamo and Makoto Demura. Characterization of a Cyanobacterial Chloride-pumping Rhodopsin and Its Conversion into a Proton Pump. 2016; 291(1):355-362. © the American Society for Biochemistry and Molecular Biology. |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/60922 |
Appears in Collections: | 生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 菊川 峰志
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