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Characterization of a Cyanobacterial Chloride-pumping Rhodopsin and Its Conversion into a Proton Pump

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/60922

Title: Characterization of a Cyanobacterial Chloride-pumping Rhodopsin and Its Conversion into a Proton Pump
Authors: Hasemi, Takatoshi Browse this author
Kikukawa, Takashi Browse this author →KAKEN DB
Kamo, Naoki Browse this author
Demura, Makoto Browse this author
Keywords: bioenergetics
membrane protein
photobiology
photoreceptor
proton pump
microbial rhodopsin
retinal proteins
Issue Date: 1-Jan-2016
Publisher: American Society for Biochemistry and Molecular Biology (ASBMB)
Journal Title: Journal of biological chemistry
Volume: 291
Issue: 1
Start Page: 355
End Page: 362
Publisher DOI: 10.1074/jbc.M115.688614
PMID: 26578511
Abstract: Light-driven ion-pumping rhodopsins are widely distributed in microorganisms and are now classified into the categories of outward H+ and Na+ pumps and an inward Cl- pump. These different types share a common protein architecture and utilize the photoisomerization of the same chromophore, retinal, to evoke photoreactions. Despite these similarities, successful pump-to-pump conversion had been confined to only the H+ pump bacteriorhodopsin, which was converted to a Cl- pump in 1995 by a single amino acid replacement. In this study we report the first success of the reverse conversion from a Cl- pump to a H+ pump. A novel microbial rhodopsin (MrHR) from the cyanobacterium Mastigocladopsis repens functions as a Cl- pump and belongs to a cluster that is far distant from the known Cl- pumps. With a single amino acid replacement, MrHR is converted to a H+ pump in which dissociable residues function almost completely in the H+ relay reactions. MrHR most likely evolved from a H+ pump, but it has not yet been highly optimized into a mature Cl- pump.
Rights: This research was originally published in Journal of Biological Chemistry. Takatoshi Hasemi, Takashi Kikukawa1, Naoki Kamo and Makoto Demura. Characterization of a Cyanobacterial Chloride-pumping Rhodopsin and Its Conversion into a Proton Pump. 2016; 291(1):355-362. © the American Society for Biochemistry and Molecular Biology.
Type: article (author version)
URI: http://hdl.handle.net/2115/60922
Appears in Collections:生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 菊川 峰志

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