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Endogenous 5-HT outflow from chicken aorta by 5-HT uptake inhibitors and amphetamine derivatives
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| Title: | Endogenous 5-HT outflow from chicken aorta by 5-HT uptake inhibitors and amphetamine derivatives |
| Authors: | Delgermurun, Dugar Browse this author | | Ito, Shigeo Browse this author →KAKEN DB | | Ohta, Toshio Browse this author →KAKEN DB | | Yamaguchi, Soichiro Browse this author →KAKEN DB | | Otsuguro, Ken-ichi Browse this author →KAKEN DB |
| Keywords: | 5-HT outflow | | amphetamine derivative | | chicken | | thoracic aorta | | uptake inhibitor |
| Issue Date: | Jan-2016 |
| Publisher: | 公益社団法人 日本獣医学会 |
| Journal Title: | Journal of veterinary medical science |
| Volume: | 78 |
| Issue: | 1 |
| Start Page: | 71 |
| End Page: | 76 |
| Publisher DOI: | 10.1292/jvms.15-0146 |
| Abstract: | Chemoreceptor cells aggregating in clusters in the chicken thoracic aorta contain 5-hydroxytryptamine (5-HT) and have voltage dependent ion channels and nicotinic acetylcholine receptors, which are characteristics typically associated with neurons. The aim of the present study was to investigate the effects of 5-HT uptake inhibitors, fluvoxamine, fluoxetine and clomipramine (CLM), and amphetamine derivatives, p-chloroamphetamine (PCA) and methamphetamine (MET), on endogenous 5-HT outflow from the isolated chick thoracic aorta in vitro. 5-HT was measured by using a HPLC system with electrochemical detection. The amphetamine derivatives and 5-HT uptake inhibitors caused concentration-dependent increases in endogenous 5-HT outflow. PCA was about ten times more effective in eliciting 5-HT outflow than MET. The 5-HT uptake inhibitors examined had similar potency for 5-HT outflow. PCA and CLM increased 5-HT outflow in a temperature-dependent manner. The outflow of 5-HT induced by PCA or 5-HT uptake inhibitors was independent of extracellular Ca2+ concentration. The 5-HT outflow induced by CLM, but not that by PCA, was dependent on the extracellular NaCl concentration. These results suggest that the 5-HT uptake system of 5-HT-containing chemoreceptor cells in the chicken thoracic aorta has characteristics similar to those of 5-HT-containing neurons in the mammalian central nervous system (CNS). |
| Rights: | http://creativecommons.org/licenses/by-nc-nd/3.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/60927 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 乙黒 兼一
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