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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
Peer-reviewed Journal Articles, etc >
Characteristics of trypsins from the viscera of true sardine (Sardinops melanostictus) and the pyloric ceca of arabesque greenling (Pleuroprammus azonus)
| Title: | Characteristics of trypsins from the viscera of true sardine (Sardinops melanostictus) and the pyloric ceca of arabesque greenling (Pleuroprammus azonus) |
| Authors: | Kishimura, Hideki1 Browse this author →KAKEN DB | | Hayashi, Kenji Browse this author | | Miyashita, Yusuke Browse this author | | Nonami, Yoshiyuki Browse this author |
| Authors(alt): | 岸村, 栄毅1 |
| Keywords: | Japanese sardine | | Sardinops melanostictus | | Arabesque greenling | | Pleuroprammus azonus | | N-terminal amino acid sequence | | Viscera | | Pyloric cecum | | Trypsin |
| Issue Date: | Jul-2006 |
| Publisher: | Elsevier |
| Journal Title: | Food Chemistry |
| Volume: | 97 |
| Issue: | 1 |
| Start Page: | 65 |
| End Page: | 70 |
| Publisher DOI: | 10.1016/j.foodchem.2005.03.008 |
| Abstract: | Trypsins, TR-S and TR-P, from the viscera of true sardine (Sardinops melanostictus) and from the pyloric ceca of arabesque greenling (Pleuroprammus azonus), respectively, were purified by gel filtration and anion-exchange chromatography. Final enzyme preparations were nearly homogeneous in sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) and the molecular weights of both enzymes were estimated to be 24,000 Da by SDS–PAGE. The N-terminal amino acid sequences of the TR-S, IVGGYECKAYSQPWQVSLNS, and TR-P, IVGGYECTPHTQAHQVSLNS, were found. The TR-S and TR-P had maximal activities at around pH 8.0 for hydrolysis of Nα-p-tosyl-l-arginine methyl ester. Optimum temperature of the TR-S and TR-P were 60 and 50 °C, respectively. The TR-S and TR-P were unstable at above 50 and 30 °C, respectively, and below pH 5.0. Both TR-S and TR-P were stabilized by calcium ion. |
| Relation: | http://www.sciencedirect.com/science/journal/03088146 |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/6113 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 岸村 栄毅
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