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Release of acid phosphatase from extraradical hyphae of arbuscular mycorrhizal fungusRhizophagus clarus
| Title: | Release of acid phosphatase from extraradical hyphae of arbuscular mycorrhizal fungusRhizophagus clarus |
| Authors: | Sato, Takumi Browse this author | | Ezawa, Tatsuhiro Browse this author →KAKEN DB | | Chenga, Weiguo Browse this author | | Keitaro, Tawaraya Browse this author |
| Keywords: | arbuscular mycorrhizal fungi | | hyphal exudate | | soil solution | | acid phosphatase | | compartment pot |
| Issue Date: | Mar-2015 |
| Publisher: | Taylor & Francis |
| Journal Title: | Soil Science and Plant Nutrition |
| Volume: | 61 |
| Issue: | 2 |
| Start Page: | 269 |
| End Page: | 274 |
| Publisher DOI: | 10.1080/00380768.2014.993298 |
| Abstract: | Arbuscular mycorrhizal (AM) fungi enhance plant uptake of available phosphorus (P) from soil through their extraradical hyphae. The mechanism underlying this P uptake enhanced by AM fungi is the increase in the surface area for absorption of available P. Little is known about utilization of unavailable P by AM fungi. We investigated whether extraradical hyphae of AM fungi release acid phosphatase (ACP). Sterilized Andosol was packed in pots that were separated into the mycorrhizal and hyphal compartments with a nylon net of 30-μm pore size. Seeds of Allium fistulosum L. were inoculated or uninoculated with the AM fungus Rhizophagus clarus (Nicolson & Schenck) Walker & Schüßler. Mullite ceramic tubes were buried in the soil of each compartment, and soil solution was collected. A. fistulosum L. and Linum usitatissimum L. inoculated with R. clarus were grown in sand culture and in vitro monoxenic culture, respectively. Uninoculated A. fistulosum L was grown in hydroponic culture to collect root exudate. The soil solution, hyphal extracts, root extract and root exudates were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis. Shoot P concentration, shoot P content and shoot dry weight were higher in the inoculated treatment than in the uninoculated treatment. Activity staining of the gel revealed that ACP activity at 187 kDa was observed in the soil solution in the inoculation treatment, and in the hyphal extract collected from sand culture and in vitro monoxenic culture, but neither in the root exudate of non-mycorrhizal plant grown in the hydroponic culture nor in the root extracts irrespective of mycorrhizal status. Those results provide strong evidence that the corresponding activity in the soil solutions in soil culture is of R. clarus CK001 origin. These findings suggest that the fungus releases ACP from extraradical hyphae into the hyphosphere. |
| Rights: | This is an Accepted Manuscript of an article published by Taylor & Francis in Soil science and plant nutrition on [date of publication], available online: http://www.tandfonline.com/10.1080/00380768.2014.993298 |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/61161 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 江澤 辰広
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