HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
Peer-reviewed Journal Articles, etc >

Release of acid phosphatase from extraradical hyphae of arbuscular mycorrhizal fungusRhizophagus clarus

Files in This Item:
Sato_SSPN.pdf356.98 kBPDFView/Open
Please use this identifier to cite or link to this item:

Title: Release of acid phosphatase from extraradical hyphae of arbuscular mycorrhizal fungusRhizophagus clarus
Authors: Sato, Takumi Browse this author
Ezawa, Tatsuhiro Browse this author →KAKEN DB
Chenga, Weiguo Browse this author
Keitaro, Tawaraya Browse this author
Keywords: arbuscular mycorrhizal fungi
hyphal exudate
soil solution
acid phosphatase
compartment pot
Issue Date: Mar-2015
Publisher: Taylor & Francis
Journal Title: Soil Science and Plant Nutrition
Volume: 61
Issue: 2
Start Page: 269
End Page: 274
Publisher DOI: 10.1080/00380768.2014.993298
Abstract: Arbuscular mycorrhizal (AM) fungi enhance plant uptake of available phosphorus (P) from soil through their extraradical hyphae. The mechanism underlying this P uptake enhanced by AM fungi is the increase in the surface area for absorption of available P. Little is known about utilization of unavailable P by AM fungi. We investigated whether extraradical hyphae of AM fungi release acid phosphatase (ACP). Sterilized Andosol was packed in pots that were separated into the mycorrhizal and hyphal compartments with a nylon net of 30-μm pore size. Seeds of Allium fistulosum L. were inoculated or uninoculated with the AM fungus Rhizophagus clarus (Nicolson & Schenck) Walker & Schüßler. Mullite ceramic tubes were buried in the soil of each compartment, and soil solution was collected. A. fistulosum L. and Linum usitatissimum L. inoculated with R. clarus were grown in sand culture and in vitro monoxenic culture, respectively. Uninoculated A. fistulosum L was grown in hydroponic culture to collect root exudate. The soil solution, hyphal extracts, root extract and root exudates were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis. Shoot P concentration, shoot P content and shoot dry weight were higher in the inoculated treatment than in the uninoculated treatment. Activity staining of the gel revealed that ACP activity at 187 kDa was observed in the soil solution in the inoculation treatment, and in the hyphal extract collected from sand culture and in vitro monoxenic culture, but neither in the root exudate of non-mycorrhizal plant grown in the hydroponic culture nor in the root extracts irrespective of mycorrhizal status. Those results provide strong evidence that the corresponding activity in the soil solutions in soil culture is of R. clarus CK001 origin. These findings suggest that the fungus releases ACP from extraradical hyphae into the hyphosphere.
Rights: This is an Accepted Manuscript of an article published by Taylor & Francis in Soil science and plant nutrition on [date of publication], available online:
Type: article (author version)
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 江澤 辰広

Export metadata:

OAI-PMH ( junii2 , jpcoar_1.0 )

MathJax is now OFF:


 - Hokkaido University