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The responses of macrophages in interaction with neutrophils that undergo NETosis
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Title: | The responses of macrophages in interaction with neutrophils that undergo NETosis |
Authors: | Nakazawa, Daigo Browse this author | Shida, Haruki Browse this author | Kusunoki, Yoshihiro Browse this author | Miyoshi, Arina Browse this author | Nishio, Saori Browse this author →KAKEN DB | Tomaru, Utano Browse this author →KAKEN DB | Atsumi, Tatsuya Browse this author →KAKEN DB | Ishizu, Akihiro Browse this author →KAKEN DB |
Keywords: | Neutrophil extracellular traps | NETosis | Macrophages | Extracellular DNA |
Issue Date: | Feb-2016 |
Publisher: | Elsevier |
Journal Title: | Journal of autoimmunity |
Volume: | 67 |
Start Page: | 19 |
End Page: | 28 |
Publisher DOI: | 10.1016/j.jaut.2015.08.018 |
PMID: | 26347075 |
Abstract: | Neutrophil extracellular traps (NETs) are net-like chromatin fibers decorated with antimicrobial proteins, which are released from dying neutrophils. The death of neutrophils with NET formation is called NETosis. Although NETs play important roles in the innate immunity, especially in the elimination of microbes, the extracellular release of DNA and intra-cytoplasmic/nuclear proteins can, on the other hand, result in diverse adversities to the hosts. Therefore, NETosis is adequately regulated in vivo. Currently, two mechanisms, namely DNase I-dependent digestion and phagocytosis by macrophages, have been shown as such regulatory mechanisms. In this study, we focused on the interaction of macrophages and neutrophils that underwent NETosis. Results demonstrated that macrophages displayed a phenotype dependent response after degradation of NETs. Several hours after the interaction, M2 macrophages induced a pro-inflammatory response, while M1 macrophages underwent cell death with nuclear decondensation. This nuclear decondensation of M1 macrophages occurred in a peptidylarginine deiminase 4-dependent manner and resulted in a local release of extracellular DNA. Thereafter, M1 macrophages degraded DNA derived from themselves in a caspase-activated DNase-dependent manner resulting in the clearance of extracellular DNA within 24 h. This transient increase and subsequent clearance mechanism of extracellular DNA seems very reasonable in terms of the double-edged sword-like property of NETs. The collective findings demonstrate a novel phenotype- and time-dependent regulation of NETosis by macrophages. (C) 2015 The Authors. Published by Elsevier Ltd. |
Rights: | ©2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0 | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
Type: | article |
URI: | http://hdl.handle.net/2115/61171 |
Appears in Collections: | 保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 石津 明洋
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