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Truncated yet functional viral protein produced via RNA polymerase slippage implies underestimated coding capacity of RNA viruses

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Title: Truncated yet functional viral protein produced via RNA polymerase slippage implies underestimated coding capacity of RNA viruses
Authors: Hagiwara-Komoda, Yuka Browse this author
Choi, Sun Hee Browse this author
Sato, Masanao Browse this author
Atsumi, Go Browse this author
Abe, Junya Browse this author
Fukuda, Junya Browse this author
Honjo, Mie N. Browse this author
Nagano, Atsushi J. Browse this author
Komoda, Keisuke Browse this author
Nakahara, Kenji S. Browse this author →KAKEN DB
Uyeda, Ichiro Browse this author →KAKEN DB
Naito, Satoshi Browse this author →KAKEN DB
Issue Date: 23-Feb-2016
Publisher: Nature Publishing Group
Journal Title: Scientific reports
Volume: 6
Publisher DOI: 10.1038/srep21411
Abstract: RNA viruses use various strategies to condense their genetic information into small genomes. Potyviruses not only use the polyprotein strategy, but also embed an open reading frame, pipo, in the P3 cistron in the -1 reading frame. PIPO is expressed as a fusion protein with the N-terminal half of P3 (P3N-PIPO) via transcriptional slippage of viral RNA-dependent RNA polymerase (RdRp). We herein show that clover yellow vein virus (ClYVV) produces a previously unidentified factor, P3N-ALT, in the +1 reading frame via transcriptional slippage at a conserved G(1-2)A(6-7) motif, as is the case for P3N-PIPO. The translation of P3N-ALT terminates soon, and it is considered to be a C-terminal truncated form of P3. In planta experiments indicate that P3N-ALT functions in cell-to-cell movement along with P3N-PIPO. Hence, all three reading frames are used to produce functional proteins. Deep sequencing of ClYVV RNA from infected plants endorses the slippage by viral RdRp. Our findings unveil a virus strategy that optimizes the coding capacity.
Type: article
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 中原 健二

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