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Chlorophyll b degradation by chlorophyll b reductase under high-light conditions
| Title: | Chlorophyll b degradation by chlorophyll b reductase under high-light conditions |
| Authors: | Sato, Rei Browse this author | | Ito, Hisashi Browse this author | | Tanaka, Ayumi Browse this author |
| Keywords: | Chlorophyll b reductase | | Light-harvesting complex | | High-light conditions | | Arabidopsis |
| Issue Date: | 21-Apr-2015 |
| Publisher: | Springer |
| Journal Title: | Photosynthesis research |
| Volume: | 126 |
| Issue: | 2 |
| Start Page: | 249 |
| End Page: | 259 |
| Publisher DOI: | 10.1007/s11120-015-0145-6 |
| PMID: | 25896488 |
| Abstract: | The light-harvesting chlorophyll a/b binding protein complex of photosystem II (LHCII) is the main antenna complex of photosystem II (PSII). Plants change their LHCII content depending on the light environment. Under high-light conditions, the content of LHCII should decrease because over-excitation damages the photosystem. Chlorophyll b is indispensable for accumulating LHCII, and chlorophyll b degradation induces LHCII degradation. Chlorophyll b degradation is initiated by chlorophyll b reductase (CBR). In land plants, NON-YELLOW COLORING 1 (NYC1) and NYC1-Like (NOL) are isozymes of CBR. We analyzed these mutants to determine their functions under high-light conditions. During high-light treatment, the chlorophyll a/b ratio was stable in the wild-type (WT) and nol plants, and the LHCII content decreased in WT plants. The chlorophyll a/b ratio decreased in the nyc1 and nyc1/nol plants, and a substantial degree of LHCII was retained in nyc1/nol plants after the high-light treatment. These results demonstrate that NYC1 degrades the chlorophyll b on LHCII under high-light conditions, thus decreasing the LHCII content. After the high-light treatment, the maximum quantum efficiency of the PSII photochemistry was lower in nyc1 and nyc1/nol plants than in WT and nol plants. A larger light-harvesting system would damage PSII in nyc1 and nyc1/nol plants. The fluorescence spectroscopy of the leaves indicated that photosystem I was also damaged by the excess LHCII in nyc1/nol plants. These observations suggest that chlorophyll b degradation by NYC1 is the initial reaction for the optimization of the light-harvesting capacity under high-light conditions. |
| Rights: | The final publication is available at Springer via http://dx.doi.org/10.1007/s11120-015-0145-6 |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/61378 |
| Appears in Collections: | 低温科学研究所 (Institute of Low Temperature Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 伊藤 寿
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