HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Institute of Low Temperature Science >
Peer-reviewed Journal Articles, etc >

Chlorophyll b degradation by chlorophyll b reductase under high-light conditions

Files in This Item:
ito.pdf581.12 kBPDFView/Open
Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/61378

Title: Chlorophyll b degradation by chlorophyll b reductase under high-light conditions
Authors: Sato, Rei Browse this author
Ito, Hisashi Browse this author
Tanaka, Ayumi Browse this author
Keywords: Chlorophyll b reductase
Light-harvesting complex
High-light conditions
Arabidopsis
Issue Date: 21-Apr-2015
Publisher: Springer
Journal Title: Photosynthesis research
Volume: 126
Issue: 2
Start Page: 249
End Page: 259
Publisher DOI: 10.1007/s11120-015-0145-6
PMID: 25896488
Abstract: The light-harvesting chlorophyll a/b binding protein complex of photosystem II (LHCII) is the main antenna complex of photosystem II (PSII). Plants change their LHCII content depending on the light environment. Under high-light conditions, the content of LHCII should decrease because over-excitation damages the photosystem. Chlorophyll b is indispensable for accumulating LHCII, and chlorophyll b degradation induces LHCII degradation. Chlorophyll b degradation is initiated by chlorophyll b reductase (CBR). In land plants, NON-YELLOW COLORING 1 (NYC1) and NYC1-Like (NOL) are isozymes of CBR. We analyzed these mutants to determine their functions under high-light conditions. During high-light treatment, the chlorophyll a/b ratio was stable in the wild-type (WT) and nol plants, and the LHCII content decreased in WT plants. The chlorophyll a/b ratio decreased in the nyc1 and nyc1/nol plants, and a substantial degree of LHCII was retained in nyc1/nol plants after the high-light treatment. These results demonstrate that NYC1 degrades the chlorophyll b on LHCII under high-light conditions, thus decreasing the LHCII content. After the high-light treatment, the maximum quantum efficiency of the PSII photochemistry was lower in nyc1 and nyc1/nol plants than in WT and nol plants. A larger light-harvesting system would damage PSII in nyc1 and nyc1/nol plants. The fluorescence spectroscopy of the leaves indicated that photosystem I was also damaged by the excess LHCII in nyc1/nol plants. These observations suggest that chlorophyll b degradation by NYC1 is the initial reaction for the optimization of the light-harvesting capacity under high-light conditions.
Rights: The final publication is available at Springer via http://dx.doi.org/10.​1007/​s11120-015-0145-6
Type: article (author version)
URI: http://hdl.handle.net/2115/61378
Appears in Collections:低温科学研究所 (Institute of Low Temperature Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 伊藤 寿

Export metadata:

OAI-PMH ( junii2 , jpcoar )

MathJax is now OFF:


 

 - Hokkaido University