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Augmin shapes the anaphase spindle for efficient cytokinetic furrow ingression and abscission

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Title: Augmin shapes the anaphase spindle for efficient cytokinetic furrow ingression and abscission
Authors: Uehara, Ryota Browse this author →KAKEN DB
Kamasaki, Tomoko Browse this author
Hiruma, Shota Browse this author
Poser, Ina Browse this author
Yoda, Kinya Browse this author →KAKEN DB
Yajima, Junichiro Browse this author
Gerlich, Daniel W. Browse this author
Goshima, Gohta Browse this author →KAKEN DB
Issue Date: 1-Mar-2016
Publisher: American Society for Cell Biology
Journal Title: Molecular biology of the cell
Volume: 27
Issue: 5
Start Page: 812
End Page: 827
Publisher DOI: 10.1091/mbc.E15-02-0101
Abstract: During anaphase, distinct populations of microtubules (MTs) form by either centrosome-dependent or augmin-dependent nucleation. It remains largely unknown whether these different MT populations contribute distinct functions to cytokinesis. Here we show that augmin-dependent MTs are required for the progression of both furrow ingression and abscission. Augmin depletion reduced the accumulation of anillin, a contractile ring regulator at the cell equator, yet centrosomal MTs were sufficient to mediate RhoA activation at the furrow. This defect in contractile ring organization, combined with incomplete spindle pole separation during anaphase, led to impaired furrow ingression. During the late stages of cytokinesis, astral MTs formed bundles in the intercellular bridge, but these failed to assemble a focused midbody structure and did not establish tight linkage to the plasma membrane, resulting in furrow regression. Thus augmin-dependent acentrosomal MTs and centrosomal MTs contribute to nonredundant targeting mechanisms of different cytokinesis factors, which are required for the formation of a functional contractile ring and midbody.
Type: article
Appears in Collections:創成研究機構 (Creative Research Institution) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 上原 亮太

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