|
Hokkaido University Collection of Scholarly and Academic Papers >
Central Institute of Isotope Science >
Peer-reviewed Journal Articles, etc >
Imaging Mass Spectrometry Reveals Acyl-Chain- and Region-Specific Sphingolipid Metabolism in the Kidneys of Sphingomyelin Synthase 2-Deficient Mice
This item is licensed under:Creative Commons Attribution 4.0 International
| Title: | Imaging Mass Spectrometry Reveals Acyl-Chain- and Region-Specific Sphingolipid Metabolism in the Kidneys of Sphingomyelin Synthase 2-Deficient Mice |
| Authors: | Sugimoto, Masayuki Browse this author | | Wakabayashi, Masato Browse this author | | Shimizu, Yoichi Browse this author →KAKEN DB | | Yoshioka, Takeshi Browse this author | | Higashino, Kenichi Browse this author | | Numata, Yoshito Browse this author | | Okuda, Tomohiko Browse this author | | Zhao, Songji Browse this author →KAKEN DB | | Sakai, Shota Browse this author | | Igarashi, Yasuyuki Browse this author →KAKEN DB | | Kuge, Yuji Browse this author →KAKEN DB |
| Issue Date: | 24-Mar-2016 |
| Publisher: | PLOS |
| Journal Title: | PLoS ONE |
| Volume: | 11 |
| Issue: | 3 |
| Start Page: | e0152191 |
| Publisher DOI: | 10.1371/journal.pone.0152191 |
| Abstract: | Obesity was reported to cause kidney injury by excessive accumulation of sphingolipids such as sphingomyelin and ceramide. Sphingomyelin synthase 2 (SMS2) is an important enzyme for hepatic sphingolipid homeostasis and its dysfunction is considered to result in fatty liver disease. The expression of SMS2 is also high in the kidneys. However, the contribution of SMS2 on renal sphingolipid metabolism remains unclear. Imaging mass spectrometry is a powerful tool to visualize the distribution and provide quantitative data on lipids in tissue sections. Thus, in this study, we analyzed the effects of SMS2 deficiency on the distribution and concentration of sphingomyelins in the liver and kidneys of mice fed with a normal-diet or a high-fat-diet using imaging mass spectrometry and liquid chromatography/electrospray ionization-tandem mass spectrometry. Our study revealed that high-fat-diet increased C18-C22 sphingomyelins, but decreased C24-sphingomyelins, in the liver and kidneys of wild-type mice. By contrast, SMS2 deficiency decreased C18-C24 sphingomyelins in the liver. Although a similar trend was observed in the whole-kidneys, the effects were minor. Interestingly, imaging mass spectrometry revealed that sphingomyelin localization was specific to each acyl-chain length in the kidneys. Further, SMS2 deficiency mainly decreased C22-sphingomyelin in the renal medulla and C24-sphingomyelins in the renal cortex. Thus, imaging mass spectrometry can provide visual assessment of the contribution of SMS2 on acyl-chain- and region-specific sphingomyelin metabolism in the kidneys. |
| Rights: | http://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/61485 |
| Appears in Collections: | アイソトープ総合センター (Central Institute of Isotope Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 久下 裕司
|
