HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
医学研究院・医学院  >
雑誌発表論文等  >

Regulated C-C motif ligand 2 (CCL2) in luteal cells contributes to macrophage infiltration into the human corpus luteum during luteolysis

manuscript.pdf13.38 MBPDF見る/開く

タイトル: Regulated C-C motif ligand 2 (CCL2) in luteal cells contributes to macrophage infiltration into the human corpus luteum during luteolysis
著者: Nio-Kobayashi, Junko 著作を一覧する
Kudo, Masataka 著作を一覧する
Sakuragi, Noriaki 著作を一覧する
Kimura, Shunsuke 著作を一覧する
Iwanaga, Toshihiko 著作を一覧する
Duncan, W. Colin 著作を一覧する
キーワード: macrophage
human corpus luteum
発行日: 2015年 8月
出版者: Oxford University Press
誌名: Molecular human reproduction
巻: 21
号: 8
開始ページ: 645
終了ページ: 654
出版社 DOI: 10.1093/molehr/gav028
抄録: Intense macrophage infiltration is observed during luteolysis in various animals including women; however, we still do not know how macrophage infiltration into the human corpus luteum (CL) during luteolysis is regulated. In this study, we examined the expression, localization and regulation of an important chemokine for the recruitment of monocyte/macrophage lineages, C-C motif ligand 2 (CCL2), in the human CL across the luteal phase and in cultured human luteinized granulosa cells (LGCs), with special reference to the number of infiltrating macrophages and luteal cell function. CCL2 mRNA increased in the non-functional regressing CL during menstruation (P < 0.01), corresponding to an elevated mRNA expression of a macrophage-derived cytokine, tumor necrosis factor (TNF), and an increased number of infiltrating macrophages positively stained with a macrophage marker, CD68. CCL2 protein was immunohistochemically localized to the cytoplasm of granulosa-lutein and theca-lutein cells, and CCL2 mRNA was significantly reduced by hCG both in vivo (P < 0.05) and in vitro (P < 0.01). CCL2 was also down-regulated by luteotrophic prostaglandin (PG) E (P < 0.0001), but up-regulated by luteolytic PGF (P < 0.05) in vitro. Administration of TNF significantly enhanced the CCL2 mRNA expression in cultured LGCs (P < 0.01). A greater abundance of infiltrating macrophages were found around granulosa-lutein cells lacking 3 beta-HSD or PGE synthase (PGES) immunostaining. CCL2 mRNA expression was negatively correlated with both HSD3B1 and PGES, suggesting that locally produced progesterone and PGE suppress macrophage infiltration into the CL. Taken together, the infiltration of macrophages in the human CL is regulated by endocrine and paracrine molecules via regulation of the CCL2 expression in luteal cells.
記述: Supplementary data are available at
Description URI:
Rights: This is a pre-copyedited, author-produced PDF of an article accepted for publication in Molecular Human Reproduction following peer review. The version of record "Mol. Hum. Reprod. (2015) 21 (8): 645-654" is available online at:
資料タイプ: article (author version)
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 小林 純子


本サイトに関するご意見・お問い合わせは repo at へお願いします。 - 北海道大学