|
Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Health Sciences / Faculty of Health Sciences >
Peer-reviewed Journal Articles, etc >
Circulating Neutrophil Extracellular Trap Levels in Well-Controlled Type 2 Diabetes and Pathway Involved in Their Formation Induced by High-Dose Glucose
This item is licensed under:Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
| Title: | Circulating Neutrophil Extracellular Trap Levels in Well-Controlled Type 2 Diabetes and Pathway Involved in Their Formation Induced by High-Dose Glucose |
| Authors: | Miyoshi, Arina Browse this author | | Yamada, Mai Browse this author | | Shida, Haruki Browse this author | | Nakazawa, Daigo Browse this author | | Kusunoki, Yoshihiro Browse this author | | Nakamura, Akinobu Browse this author →KAKEN DB | | Miyoshi, Hideaki Browse this author →KAKEN DB | | Tomaru, Utano Browse this author →KAKEN DB | | Atsumi, Tatsuya Browse this author →KAKEN DB | | Ishizu, Akihiro Browse this author →KAKEN DB |
| Keywords: | Aldose reductase inhibitor | | Microvascular complications | | Myeloperoxidase-DNA complex | | Neutrophil extracellular trap | | Polyol pathway | | Type 2 diabetes |
| Issue Date: | 2016 |
| Publisher: | Karger |
| Journal Title: | Pathobiology |
| Volume: | 83 |
| Issue: | 5 |
| Start Page: | 243 |
| End Page: | 251 |
| Publisher DOI: | 10.1159/000444881 |
| PMID: | 27189166 |
| Abstract: | Objectives: Although intensive therapy for type 2 diabetes (T2D) prevents microvascular complications, 10% of well-controlled T2D patients develop microangiopathy. Therefore, the identification of risk markers for microvascular complications in well-controlled T2D patients is important. Recent studies have demonstrated that high-dose glucose induces neutrophil extracellular trap (NET) formation, which can be a risk for microvascular disorders. Thus, we attempted to determine the correlation of circulating NET levels with clinical/laboratory parameters in well-controlled T2D patients and to reveal the mechanism of NET formation induced by high-dose glucose. Methods: Circulating NET levels represented by myeloperoxidase (MPO)-DNA complexes in the serum of 11 well-controlled T2D patients and 13 healthy volunteers were determined by enzyme-linked immunosorbent assay. The pathway involved in the NET formation induced by high-dose glucose was determined using specific inhibitors. Results: Serum MPO-DNA complex levels were significantly higher in some well-controlled T2D patients in correlation with the clinical/laboratory parameters which have been regarded as risk markers for microvascular complications. The aldose reductase inhibitor, ranirestat, could inhibit the NET formation induced by high-dose glucose. Conclusions: Elevated levels of circulating NETs can be a risk marker for microvascular complications in well-controlled T2D patients. The polyol pathway is involved in the NET formation induced by high-dose glucose. |
| Rights: | © 2016 The Author(s) Published by S. Karger AG, Basel | | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/61981 |
| Appears in Collections: | 保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 石津 明洋
|
